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摘 要：为探讨细胞骨架在肺抗原呈递细胞和其它细胞的表达，用细胞骨架的单克隆抗体对日本正常雌兔肺切片进行免疫组织化学ABC法染色。结果表明，肌动蛋白(actin)表达于血管、肺内导气部管壁的平滑肌；微管蛋白(tubulin)和角蛋白-7(cytokeration-8)表达于Clara细胞、肺泡Ⅰ型和Ⅱ型上皮细胞以及肺巨噬细胞；波形蛋白(vimentin)表达于肺血管内皮和肺巨噬细胞。本文对细胞骨架的意义进行了讨论。
The Expression of the Cytoskeleton Protein of the Lung in Rabbit
wang Xiuqin,Sun Haimei,Zeng Xiaobei(Department of Histology and Embryology, Capital University of Medical Sciences, Beijing 100054)
hieohiko Iwatsuki,Masumi Suda(Department of Anatomy, Kawasaki Medical School, Kuraahiki 70-0192, Japan)
Abstract：To investigate the distribution of cytoskeletal components in rabbit, the lung of normal female adult white rabbits were fixed well in 4% 40 paraformaldehyde and paraffin section were prepared accoridng to routine methods. Anticytokeratin-7, -8, -18, -19, antiactin, antivimentin and anti-alpha-tubulin monochlonae antibodies, were employed with immunohistochemical ABC methed. Resalts: The clara ceils in terminal and respiratory bronchioles were immunopositive for cytokeratin-7, vimentin and alpha-tubulin. The type Ⅰ and type Ⅱ pulmonary alveolar cells show positive reactivity for cytokeratin-7 and alpha-tubulin. The alveolar macrophage shows cytokeratin-7, vimentinand alpha-tubulin positive. The smooth muscle cells in small bronchus, terminal and respiratory bronchioles and alveolar duct show actin positive. Significance of expression of these cytoskeletal proteins in lung was discussed.
Keywords：cytoskeleton; lung; immunohistochemistry; rabbit参考文献：
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