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Received 19 March 2003/ Returned for modification 7 May 2003/ Accepted 19 May 2003
ABSTRACT |
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We recently demonstrated by DNA-DNA hybridization that the putative R-M system NmeDI is specific to these two lineages (5, 7). The R-M system was inserted between the pheS and the pheT genes, and other meningococcal lineages harbored different genes at this location. The MLST reference strain collection (9), including 103 mostly pathogenic isolates, was recently used to demonstrate the specificity of NmeDI for the ST-8 and ST-11 complexes (5), which was 95% (there were four false positives and 83 true negatives; specificity was calculated by using the formula specificity = true negatives/[false positives + true negatives]). We reevaluated this specificity by using the Bavarian meningococcal carrier strain collection (822 isolates, 322 STs), for which a complete MLST data set was available (6). The collection comprised 10 isolates belonging to the ST-8 and ST-11 complexes, which were all positive for NmeDI upon DNA-DNA hybridization in accordance with the protocol published recently (5). Of the remaining 812 isolates, only 5 (<1%) were NmeDI positive without belonging to the ST-8 and ST-11 complexes (4 isolates of a total of 136 isolates belonging to the ST-44 complex; 1 isolate was ST-914). This finding indicates that after horizontal gene transfer, NmeDI is probably not genetically fixed in lineages other than the ST-8 and ST-11 complexes. Because of the low number of ST-8 and ST-11 complex strains, the positive predictive value (true positives/[true positives + false positives]) of DNA-DNA hybridization with NmeDI was only 67% for the Bavarian meningococcal carriage isolate collection. However, the negative predictive value (true negatives/[true negatives + false negatives]) was 100% and the specificity was 99.4%. These results prompted us to generate monoclonal antibodies (MAbs) against the NmeDI endonuclease in order to obtain an easy-to-use reagent for the detection of ST-8 and ST-11 complex meningococci. Mice were immunized with a recombinant peptide comprising amino acids 1 to 143 of NmeDI (National Center for Biotechnology Information accession no. ), and MAbs were raised in accordance with standard protocols (10). Affinity-purified MAb 3/9-2 specifically reacted with NmeDI in meningococcal lysates, as demonstrated by Western blot analysis and by dot immunoblotting. As expected, a 40-kDa protein was detected in Western blots (data not shown). The meningococcal protein was not detectable by enzyme-linked immunosorbent assay, although the recombinant peptide expressed in Escherichia coli was detectable in this assay (data not shown). Therefore, dot blot assays were used for further analyses. We suggest that the epitope in the correctly folded protein is not accessible to the antibody and that denaturing methods have to be used. Approximately 109 CFU were suspended in 1,000 µl of a sample solution containing 5% ß-mercaptoethanol and 2% sodium dodecyl sulfate (11). Ten microliters was dotted onto nitrocellulose membranes (Schleicher & Schüll, Dassel, Germany) and dried. Unspecific binding sites on the membranes were blocked by incubation with phosphate-buffered saline-5% skim milk powder-0.1% Tween 20 for 60 min. A 1:5,000 dilution in phosphate-buffered saline-1% skim milk powder-0.1% Tween 20 of the affinity-purified MAb 3/9-2 stock solution containing 2.26 mg of protein/ml was added, and the mixture was incubated for 60 min. The blots were developed as described previously (11). MAb 3/9-2 reacted with the 15 NmeDI-positive meningococcal isolates of the Bavarian isolate collection, suggesting constitutive expression. It did not react with 15 selected NmeDI-negative isolates from the same collection (Fig. 1). Therefore, the results obtained with MAb 3/9-2 correlated with the reactivity of the strains with an NmeDI probe. MAb 3/9-2 reacted with eight strains of the ST-8 complex and nine strains of the ST-11 complex, which were isolated between 1967 and 2000 in nine different countries (data not shown). This finding again underlines the association of NmeDI with these lineages. The MAb did not react with a collection of 26 genetically diverse strains of N. lactamica described recently (2; data not shown). It also did not react with a variety of phylogenetically diverse bacterial species (Table 1). However, some of the Staphylococcus aureus isolates used were positive. This reaction was due to nonspecific binding of MAb 3/9-2 by staphylococcal protein A, because the strains also bound mouse MAbs directed against the meningococcal serogroup B capsule (MAb 735) (8) and human complement factor C3 (MAb 755) (11), respectively (data not shown). In conclusion, we report on MAb 3/9-2, which specifically identified meningococci of the ST-8 and ST-11 complexes. We recommend the use of the antibody in reference laboratories for rapid assignment of isolates to the ST-8 and ST-11 complexes prior to further sequence typing. The antibody showed no cross-reactivity with other neisserial species and several other bacterial genera, with the exception of an unspecific binding to S. aureus. Use of the antibody for detection of NmeDI in clinical speci-mens would require elimination of the Fc portion of the antibody.
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ACKNOWLEDGMENTS |
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Gabi Heinze and Christine Meinhardt are thanked for expert technical assistance. Johannes Elias is thanked for help with the dot blot procedure and for critically reading the manuscript. We thank M. Achtman (Berlin, Germany), D. A. Caugant (Oslo, Norway), I. Ehrhard (Dresden, Germany), and R. Borrow (Manchester, England) for providing strains.
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