点击显示 收起
|
Abstract |
---|
Objective— The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively, and bidirectional signals mediated by both proteins play an important role in vascular development. However, how such bidirectional signals are required for cell-cell adhesion or repulsion remains unclear.
Methods and Results— Using a cell line and sorted primary endothelial cells, we show that ephrin-B2 forward signaling through the EphB4 receptor inhibits cell adhesion, whereas EphB4 reverse signaling by the transmembrane ephrin-B2 ligand does not. Cell migration is also inhibited on immobilized ephrin-B2-Fc but not on EphB4-Fc protein.
Conclusions— Ephrin-B2 forward signaling and EphB4 reverse signaling differentially affect cell adhesion and migration between arterial and venous endothelial cells.
Key Words: ephrin-B2 EphB4 cell adhesion spreading vascular development
Introduction |
---|
Top Abstract Introduction Methods Results Discussion References |
---|
The ephrins, unlike ligands for other receptor tyrosine kinases, must be membrane-anchored to activate Eph receptors.6 Based on how they are tethered to the membrane, ephrins are divided into 2 subclasses. The 5 members of the ephrin-A subclass (ephrin-A1 to -A5) are tethered via a glycosylphosphatidylinositol (GPI) anchor, whereas the 3 members of the ephrin-B subclass (ephrin-B1 to -B3) have a transmembrane domain and highly conserved cytoplasmic domains. Ephrin-B ligands interact primarily with the B subset of Eph receptors, which consist of at least 6 members.6,7 A characteristic of ephrin/Eph signaling is the potential for bidirectional signaling, that is, classical forward signaling by the Eph receptor via its intrinsic tyrosine kinase activity and reverse signaling by the transmembrane ephrin-B ligand via its cytoplasmic domain.
Recent work shows that ephrin-B2 ligand and its cognate EphB4 receptor are required for vascular development. Targeted disruption of the ephrin-B2 gene in mice leads to embryonic lethality at embryonal day (E) 10.5, attributable to a defect in both arterial and venous vessel remodeling.8,9 This defect is accompanied by a failure of intercalation between arteries and veins. EphB4 homozygous mutants have a similar phenotype with ephrin-B2 homozygous embryos in the vascular system.10 Interestingly, ephrin-B2 and EphB4 display a remarkable reciprocal expression pattern in endothelial tissue. Ephrin-B2 marks arterial endothelial cells, whereas EphB4 marks venous endothelial cells.11–13 Thus, signaling between arteries and veins mediated by ephrin-B2 and EphB4 is required for proper morphogenesis of the intervening capillary beds and network as well as for interdigitation and differential growth of arterial and venous vessels. These findings strongly indicate that ephrin-B2/EphB4 signaling plays an important role in vascular development, especially in the determination and boundary formation between arteries and veins. However, despite the remarkable reciprocal distribution of ephrin-B2 and EphB4 during early vascular development, it is not clear how interactions between ephrin-B2 and EphB4 occur in vascular development and whether ephrin-B2 forward signaling (ephrin-B2/EphB4 signaling) and EphB4 reverse signaling (EphB4/ephrin-B2 signaling) result in different functions. Here we demonstrate that there are differences in ephrin-B2 forward signaling and EphB4 reverse signaling occurring in cell-cell interaction between endothelial cells.
Methods |
---|
Top Abstract Introduction Methods Results Discussion References |
---|
Cell Adhesion Assay
Petri dishes (35 mm, Falcon) were coated with a layer of nitrocellulose (Schleicher & Schuell), as described previously.16 After washing, dishes were incubated with PBS(-) containing ephrin-B2-Fc, EphB4-Fc, and CD4-Fc or human immunoglobulin (Sigma-Aldrich) as control Fc protein for 2 hours before assay. Wells were washed with PBS(-) twice and blocked at 37°C with 1% BSA for 30 minutes. b-End3 cells were harvested with cell dissociation buffer (Gibco) or 1 mmol/L EDTA, washed twice in medium containing 1% BSA, and plated at a density of 1x105 cells per well. After incubation at 37°C for 1 hour (or as specified), unattached cells were dislodged by washing with PBS(-) 3 times. The data from 3 independent experiments performed in triplicate are displayed as the fold of immobilized control-Fc protein.
Fc Protein Binding Assay
For the Fc protein assay, b-End3 cells were gently detached from dishes with EDTA. After cells were washed with PBS(-)/5% FCS twice, they were incubated with 2 µg/mL Fc-fusion protein for 30 minutes on ice. After washing with PBS(-)/5% FCS twice, cells were incubated with FITC-conjugated goat anti-human IgG(H+L) (Immunotech). Stained cells were analyzed by a FACSCalibur (Becton Dickinson).
Reverse Transcriptase–Polymerase Chain Reaction Analysis
RNA was extracted from sorted cells by FACS analysis using an RNeasy Mini kit (Qiagen). RNA was reverse transcribed using an Advantage RT-for-PCR Kit (Clontech). cDNAs were amplified with an Advantage cDNA PCR Kit (Clontech) in a Gene AmpPCR System 9700 (Perkin-Elmer Inc) for 30 or 35 cycles. Sequences of specific primers for reverse transcriptase–polymerase chain reaction (RT-PCR) were as follows: ephrin-B2: sense: 5'-TCTGTGTGGAAGTACTGTTGGGGACTTT-3'; antisense: 5'-TGTACCAGCTTCTAGCTCTGGACGTCTT-3'; EphB2: sense: 5'-GGACGGCGAATGGCTGGTG-3'; antisense: 5'-TCGGCTTCTGTCATGGTTTGG-3'; EphB3 and EphB49: Dll4: sense: 5'-ATCTTCCAGCTGCGGCTGC-3'; antisense: 5'-GCACTCATAATGTCCGAAGT-3'; Bmx sense: 5'-GCAACATACGCTATATTCCA-3'; antisense: 5'-AAGCATGCAGATTTTCCTCT-3'; ß-actin: sense: 5'-TCGTGCGTGACATCAAAGAG-3'; antisense: 5'-TGGACAGTGAGGCCAAGATG-3'.
Each cycle consisted of 15 seconds of denaturation at 94°C and 3 minutes of annealing and extension at 68°C. PCR products were separated on a 1.5% agarose gel and stained with ethidium bromide.
Generation of an Anti-EphrinB2 Monoclonal Antibody
Rat monoclonal antibodies against ephrinB2-Fc protein were produced using standard methods. In brief, an 8-week-old Wistar rat was immunized subcutaneously with 600 µg ephrinB2-Fc protein in complete Freund’s adjuvant (Difco) and then administered 3 intraperitoneal shots of 100 µg ephrinB2-Fc protein in Freund’s incomplete adjuvant in alternating weeks. Three days after an intravenous boost of 200 µg ephrinB2-Fc protein, spleen cells were harvested and fused with murine myeloma cells, P3x63-Ag.8.U1. Undiluted supernatants from hybridomas were screened by ELISA on plates coated with 50 ng/mL ephrinB2-Fc. Positive hybridomas were cloned by the limiting dilution technique and were subcloned twice. Western blotting analysis was described previously.14
Cell Preparation and Flow Cytometry
Cell preparation conditions for FACS analysis were as described.17 In brief, after dissecting E12.5 mouse embryos, tissues were homogenized to a single-cell suspension with an 18G needle and passed through a nylon mesh screen. These tissues were incubated with 0.05% collagenase S1 (Nitta gelatin) for 2 hours at 37°C and subsequently drawn through a 23G needle. Debris and cell aggregates were removed with nylon mesh again. Single cells from tissue were stained with Cy5-labeled anti-ephrin-B2 monoclonal antibody and anti-PECAM-1/CD31-PE (MEC13.3, rat anti-mouse monoclonal; Pharmingen). Stained cells were analyzed and sorted by a FACSvantage (Becton Dickinson, ).
Immunofluorescence Microscopy
After stimulation with Fc chimeric protein, b-End3 cells were fixed with 4% paraformaldehyde/PBS, pH 7.4, for 15 minutes at room temperature and, if necessary, permeabilized with 0.2% Triton X-100/PBS. After washing, cells were incubated with 1% BSA or 5% normal goat serum/1% BSA and exposed to FITC-conjugated goat anti-human IgG (H+L) (Immunotech) for detection of immobilized Fc-fusion protein. This treatment was followed by incubation with Rhodamine Red-X–conjugated anti-mouse IgG antibody (Jackson ImmunoResearch). F-actin was detected by staining with rhodamine-conjugated phalloidin (Sigma-Aldrich). Cells were counterstained with DAPI (Dojin) to identify nuclei. Stained cells were mounted with 1,4-diazabicyclo-2,2,2-octane/glycerol. Images were obtained with fluorescent microscopy on an AX70 microscope (Olympus). Images were processed using Photoshop software (Adobe).
Cell Proliferation Assay
To determine the BrdU-labeling index, b-End3 cells were pulsed with 10 µmol/L BrdU for 1 hour. After washing with PBS(-) 3 times, BrdU incorporation was determined using a BrdU staining kit according to the manufacturer’s protocol (Oncogene research product). The colony assay was described previously.18 Two hundred b-End3 cells were plated onto 24 wells. After 7 days in culture, cells growing as small colonies were fixed and stained with 0.5% crystal violet.
Results |
---|
Top Abstract Introduction Methods Results Discussion References |
---|
|
To determine whether the ephrin-B2-EphB4 signaling induced cell adhesion in endothelial cells, we plated b-End3 cells on immobilized EphB4-Fc, ephrin-B2-Fc, or control-Fc proteins. Adherence of b-End3 cells to immobilized EphB4-Fc and control-Fc fusion proteins was equivalent to their adherence to uncoated plates, and this adhesion was unchanged by varying the concentrations of immobilized EphB4-Fc or control-Fc proteins (C). By contrast, immobilized ephrin-B2-Fc protein inhibited cell adhesion relative to controls in a dose-dependent manner (C). This result indicates that ephrin-B2 forward signal is inhibitory for adhesion to cells expressing the cognate receptor.
Differences in Signaling Between Ephrin-B2 and EphB4 in Primary Endothelial Cells
To examine adhesion or repulsion in ephrin-B2/EphB4 signaling in primary endothelial cells, we made a monoclonal antibody against murine ephrin-B2 to use for FACS analysis. After screening, Keb2 (IgM) was isolated as a clone reacting specifically to the extracellular domain of ephrin-B2 protein by ELISA. Keb2 reacted specifically to ephrin-B2-transfected BaF3 cells but not to EphB4- or only vector-transfected BaF3 cells (data not shown). Moreover, the specific reactivity of Keb2 was confirmed by the finding that an excess molar ratio of ephrin-B2-Fc proteins completely blocked the reactivity of Keb2 with ephrin-B2 expressing BaF3 cells (data not shown). Using Keb2, we analyzed the expression of ephrin-B2 on CD31-positive endothelial cells in E12.5 whole embryos. As shown in A, ephrin-B2+ CD31+ cells was expressed on 1.7% of all cells from E12.5 embryo. Approximately 1.7% and 2.1% of all cells expressed ephrin-B2+ and ephrin-B2- in CD31+ fraction in E12.5 whole embryos, respectively. Ephrin-B2 was also expressed on a subset of CD31- cells (5.3%). We examined the expression of arterial or venous specific markers by RT-PCR analysis (B). EprhinB2+ cells expressed arterial-specific markers Dll4 and Bmx,20,21 whereas ephrin-B2- fraction expressed the venous endothelial specific marker, EphB4.
|
To test whether ephrin-B2/EphB4 signaling induces cell adhesion, we examined adhesion of sorted primary cells on immobilized ephrin-B2-Fc or EphB4-Fc. Immobilized EphB4-Fc proteins induced cell adhesion of ephrin-B2+ CD31+ cells (C). Some of the adhered ephrin-B2+ CD31+ cells on EphB4-Fc proteins became flat (Cb). By contrast, immobilized ephrin-B2-Fc proteins inhibited cell adhesion of ephrin-B2- CD31+ cells (Cc). Quantitative analysis showed that the number of adhering ephrin-B2+ cells to EphB4-Fc protein was 6 times greater than those adhering to ephrin-B2 or control-Fc protein (Db). By contrast, adhesion rate of ephrin-B2- cells on ephrin-B2-Fc protein was lower than that seen on EphB4-Fc or control-Fc protein (Da). We conclude that ephrin-B2 forward signaling inhibited cell adhesion, whereas EphB4 reverse signaling enhanced cell adhesion in primary endothelial cells.
Suppression of Actin Cytoskeletal Reorganization by Ephrin-B2
To investigate potential changes in cell morphology in endothelial cells induced by ephrin-B2 and EphB4 signaling, we examined cell shape and change of actin filaments in adherent primary endothelial cells by fluorescence microscopy. Cells were fixed with 4% paraformaldehyde and stained with rhodamine-conjugated phalloidin to reveal the actin cytoskeleton. After 2 hours of plating, there was no significant difference in morphology of ephrin-B2- cells on immobilized ephrin-B2-Fc, EphB4-Fc, or control-Fc proteins (Ac). On the other hand, sprouting of ephrin-B2+ cells was induced only on immobilized EphB4-Fc (Af). After 8 hours of plating, ephrin-B2- cells spread and flattened on immobilized EphB4-Fc and control-Fc protein, and actin filaments were seen associated with membrane ruffling, which is typical of filopodia and lamellipodia (Bc). 22,23 However, ephrin-B2- cells on immobilized ephrin-B2-Fc remained round, failed to spread, and showed few organized stress fibers of actin filaments (Figure 3Bb). There was no significant difference in morphology of ephrin-B2+ cells on immobilized EphB4-Fc, ephrin-B2-Fc, or control-Fc protein (Bf). Quantitative analyses showed that cell spreading of ephrin-B2- cells was drastically inhibited on immobilized ephrin-B2-Fc protein (C). By contrast, cell spreading of ephrin-B2+ cells was induced on immobilized EphB4-Fc protein but not on ephrin-B2-Fc or control-Fc proteins (C). Ephrin-B2 forward signaling also inhibited cell spreading in b-End3 cells (data not shown). These results indicate that cell spreading was suppressed by ephrin-B2 forward signaling, whereas EphB4 reverse signaling induced cell adhesion and cell spreading in endothelial cells.
|
Ephrin-B2 Signaling Exhibits Repulsion Activities Toward Cell Migration
To additionally determine whether ephrin-B2/EphB4 bidirectional signaling can directly influence growth direction in endothelial cells, we examined how endothelial cells grew on immobilized ephrin-B2-Fc or EphB4-Fc proteins during short or long culture periods. For these experiments, small quantities of ephrin-B2-Fc or EphB4-Fc proteins were spotted on nitrocellulose-coated dishes. After 12 hours of plating, elongation of filopodia in b-End3 cells had nothing to do in the presence of immobilized EphB4-Fc protein (Aa). By contrast, immobilized ephrin-B2-Fc clearly induced the arrest of elongation of filopodia in b-End3 cells (Ab). In long culture periods, numbers of cells were drastically different between immobilized EphB4-Fc proteins and immobilized ephrin-B2-Fc (B). When b-End3 cells were plated on immobilized EphB4-Fc, b-End3 cells were evenly distributed (Ba), whereas b-End3 cells did not move across immobilized ephrin-B2-Fc protein (Bb). These data suggest the possibility that ephrin-B2 forward signaling induced cell repulsion by ephrin-B2/EphB4 interaction.
|
It was reported that ephrin-A2 forms a stable complex with the metalloprotease Kuzbanian in the absence of EphA receptors and that when ephrin-A2 ligand binds to the EphA receptor, Kuzbanian is activated and locally cleaves ephrin-A2 from the cell surface.24 To determine whether metalloproteinases are involved in the process activated by ephrin-B2 forward signaling, inhibition of cell adhesion by immobilized ephrin-B2-Fc was monitored in the presence of a metalloproteinase inhibitor, o-phenanthrolene (C). Cell adhesion and spreading were suppressed on immobilized ephrin-B2-Fc protein (Cc), whereas treatment with o-phenanthrolene significantly rescued inhibition of cell spreading in b-End3 cells (Cf). These experiments suggest the possible involvement of a metalloproteinase in the repulsion mediated by ephrin-B2 forward signaling.
Inhibition of Cell Proliferation by Ephrin-B2 Signaling
To determine how ephrin-B2/EphB4 signaling affects cell proliferation, we assayed incorporation of 5-bromodeoxyuridine (BrdU) in b-End3 cells. After culturing on immobilized Fc protein for 24 hours, b-End3 cells were treated with BrdU 1 hour before fixation and immunostained to identify cells incorporating BrdU . Treatment with immobilized ephrin-B2-Fc significantly reduced the labeling index compared with treatment with immobilized EphB4-Fc and control-Fc protein (10% versus 42%). Furthermore, to characterize the single cell proliferation against ephrin-B2/EphB4 signaling, we undertook a colony assay using endothelial cells.25 Treatment with immobilized ephrin-B2-Fc significantly reduced the size of colonies and the number of colonies formed compared with treatment with immobilized EphB4-Fc or control-Fc . These results show that ephrin-B2 forward signaling inhibits cell proliferation. Moreover, we examined the possibility that apoptosis was induced by ephrin-B2 forward signaling by staining for annexin V and propidium iodide. Treatment with immobilized ephrin-B2-Fc did not induce apoptosis compared with immobilized EphB4-Fc or control-Fc (data not shown). Taken together, these results indicated that ephrin-B2 forward signaling inhibits cell proliferation and does not induce apoptosis.
|
Discussion |
---|
Top Abstract Introduction Methods Results Discussion References |
---|
Eph receptor tyrosine kinases and their membrane-bound ephrin ligands mediate cell-cell interactions and participate in several developmental processes.4,7 Bidirectional signaling mediated by ephrins and Eph has important functions in cell-cell recognition events, including those that occur during axon pathfinding26,27 and hindbrain segmentation.28–30 Moreover, ephrin/Eph signalings have recently emerged as essential regulators of cell adhesion in neuronal and endothelial cells.5,31 We have also shown that ephrin-B2/EphB4 signaling mediates reciprocal interactions between arterial and venous endothelial cells and surrounding cells to form vessels.14 However, there is little understanding of how such bidirectional signaling acts in vascular development and network formation. Previous studies have shown that signaling from ephrin through Eph activates cell attachment or detachment.18,25 To understand this question, we analyzed the cell-cell interaction using a pro-B cell line, BaF3 transfected with ephrin-B2 or EphB4. Heterophilic cell aggregation was observed after coincubation of BaF3/ephrin-B2 cells and BaF3/EphB4 cells (data not shown). Because these cells are nonendothelial cells, next we asked whether ephrin-B2/EphB4 signaling induced cell attachment or detachment in the vascular system.
To answer this question, we analyzed the signaling of ephrin-B2/EphB4 in both the b-End3 endothelial cell line, which expresses endogenous ephrin-B2 and EphB4, and primary endothelial cells sorted from murine embryos, which were either ephrin-B2+ or EphB4+. CD31-positive cells in E12.5 embryos were clearly divided into 2 fractions by the ephrin-B2 MoAb. RT-PCR revealed that sorted ephrin-B2–positive cells express Dll4 and Bmx, whereas ephrin-B2–negative cells express EphB4. Thus, we regard the former as arterial endothelial cells and the latter as venous endothelial cells.
Ephrin-B2 forward signaling clearly induces repulsive effects in b-End3 cells and primary ephrin-B2–negative (EphB4-positive) endothelial cells. Cell spreading was inhibited on b-End3 cells and EphB4+ cells from murine embryos on immobilized ephrin-B2-Fc but not on EphB4-Fc protein. This inhibition was accompanied by inhibition focal contact formation, indicating an important role of ephrin-B2 signaling in endothelial cell-cell interaction through cytoskeletal regulation. We have demonstrated that migration of b-End3 cells across immobilized ephrin-B2-Fc was inhibited and that cell proliferation was also inhibited on ephrin-B2-Fc but not EphB4-Fc protein. Inhibition of proliferation occurred not only through the repulsive effect of ephrin-B2 but by direct suppressive effects on endothelial cells, because delayed addition of ephrin-B2 suppressed BrdU uptake. Based on these findings, our results indicate that ephrin-B2 forward signaling has an inhibitory effect on attachment, migration, and proliferation of EphB4+ cells. Recently, it was shown that EphB2 reverse signaling induced changes in cytoskeletal regulation and that the SH2/SH3 domain adapter protein Grb4 binds to the cytoplasmic domain of ephrin-B1 in a phosphotyrosine-dependent manner.32 The observation that the region of ephrin-B1 binding to Grb4 is conserved in ephrin-B2 cytoplasmic tail suggests that EphB4 reverse signaling through ephrin-B2 may be similar through EphB2 reverse signaling through ephrin-B1.
Hattori et al24 recently reported that ephrin-A2 forms a stable complex with the metalloprotease Kuzbanian in the absence of EphA. When EphA binds to ligand, Kuzbanian is activated and locally cleaves ephrin-A2 from the cell surface. Because a conserved motif, which exists at cleavage site by Kuzbanian is found in the extracellualry region of ephrin-B2, it is possible that similar mechanisms operate in the case of repulsion by ephrin-B2 forward signaling. We show here that a metalloproteinase inhibitor rescued inhibition of cell adhesion by ephrin-B2 forward signaling. However, we have not detected an ephrin-B2 cleavage fragment in this study. Therefore, it is necessary to investigate additionally the mechanisms of repulsion in ephrin-B2 forward signaling in EphB4+ cells. Furthermore Lu et al33 described a new ephrin-B interacting PDZ-containing protein, PDZ-RGS3, that regulates Rho GTPases activity. These data suggest that downstream signaling in ephrin-B2 may interact with small G protein signalings.
The difference between ephrin-B2 forward and EphB4 reverse signaling is important for spatially and temporally appropriate vascular network formation. Capillaries consist of ephrin-B2–positive cells and ephrin-B2–negative cells.12 Our findings suggest that capillary formation is preceded by artery formation. EphB4+ venous cells cannot connect to ephrin-B2+ cells, whereas ephrin-B2+ arterial cells can bind to EphB4+ cells in capillary network formation. In Xenopus embryos, disruption of EphB4 signaling or misexpression of ephrin-B ligands results in intersomitic veins growing abnormally into the adjacent somitic tissue.34 Moreover, activin receptor-like kinase-1, Notch and Gridlock, which are upstream molecules of ephrin-B2, mutant as well as mice lacking ephrin-B2 and EphB4 reported the defect of vascular network formation.35–37 Therefore, our data and those of others indicate that such mutant phenotypes are caused by disruption of cell-cell interactions involving ephrin-B2 forward and EphB4 reverse signaling during vascular network formation. These results additionally suggest that downstream signaling in endothelial cells is important for appropriate vascular network formation.
In summary, we have demonstrated that ephrin-B2 forward signaling induced cell detachment and inhibited cell spreading, migration, and proliferation, whereas EphB4 reverse signaling induced cell attachment, spreading, and migration. Our data on cell-cell interactions in endothelial cells indicates that differential functions of bidirectional signaling play an important role in vascular development, network formation, and remodeling.
Acknowledgments |
---|
Received June 10, 2002; accepted December 17, 2002.
References |
---|
Top Abstract Introduction Methods Results Discussion References |
---|