摘要:8, 1994ReagentsAshing buffer:10 g Mg(NO3)2 100 ml EtOH1。5 N HCl stock:119。7 ml concentrated HCl (11。3 ml H2O1 N Sulfuric acid stock:28。
08-02-03摘要:Gel-elongation assay for type II fatty acid synthesisSrinivas Kodali Andrew Galgoci Sheo Singh Dr。 Jun Wang Dr。, jun_wang2@merck。com, Merck Research Laboratories Related Journal Article Informa...
08-02-03摘要:Gel-elongation assay for type II fatty acid synthesisSrinivas Kodali Andrew Galgoci Sheo Singh Dr。com, Merck Research Laboratories Related Journal Article InformationManuscript: 2006-01-00990CJou...
08-02-03摘要:l of phenol red solution。-- The solution should appear yellow since the free acid has not been neutralized as yet。4) Once a pink color has been attained, the solution is “neutralized。“-- At th...
08-02-03摘要:Methylation of Fatty Acids (Kropinski Method)OBJECTIVE:To methylate fatty acids in whole cells or lipopolysaccharide。REAGENTS : Methanol-Hydrochloride Reagent Kit 10mg of whole cells or 1 mg of li...
08-02-03摘要:Phosphate AssayContributor: Jiandi ZhangDate: July 4, 19961。 Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80。 Use the screw top glass ...
08-02-03摘要:5 mmole of dioleoyl phosphatidyl choline under nitrogen in a disposable glass tube。Evacuate in dessicator under vacuum for 30 minutes。Add buffer / dH20 to required volume and scrape the sides of ...
08-02-03摘要:11, 1993Synthesis reaction:1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1。3 in 5 ml of methanol。2) Allow reaction to proceed at room temperature for atleast 18 hours in th...
08-02-03摘要:EXTRACTION AND ANALYSIS OF LIPIDSThe lipophorin molecule that we isolated from locust hemolymph functions as a re-usable lipid shuttle。08 g/ml and contains less lipids than the loaded form of the ...
08-02-03摘要:Lipoprotein Analysis -week 3During this week you will analyze the SDS PAGE and determine the protein content in the lyophilized fraction of lipoproteins gravimetrically。 You will also extract the ...
08-02-03摘要:In vitro Sphingomyelinase Assay Contributor: Suprya JayadevDate: May 25, 1993Reagents:Lysis buffer25 mM Tris-HCl, pH 7。45 mM EDTA1 mM ATP20 µ。g/ml CLAP1 mM PMSFBuffer A10 mM MgCl20。3) Resus...
08-02-03摘要:Ci/ml of 3H-galactose for 72 hours。3) Extract lipids by washing 3 times with chloroform-methanol (1:1)。--- Use 3 ml each time。4) Pool the solvents from the extractions and dry down lipids。
08-02-03摘要:DGK Membrane PreparationContributor: Suprya JayadevDate: Dec。g/ml ampicillinHigh salt bufferfor 1 L50 mM KH2PO4 6。8 g150 mM KCl 11。18 g50 mM sodium pyrophosphate (Na4P2O7-10H2O) 22。
08-02-03摘要:10, 1995Buffers:- 2X buffer10 ml 0。25 ml 1 M MgCl21。0 ml 0。6-- Bring volume up to 50 ml with distilled water。
08-02-03摘要:Desalting Acidic GlycosphingolipidsContributor: Suprya JayadevDate: November 10, 19931) Prepare a C-18 reverse phase prep-sep cartridge by washing with:a) 10 ml of methanolb) 20 ml of chloroform-me...
08-02-03摘要:11, 1991Packing column:1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform。3) Pour gel solution into column and use a stir bar to press out any bubbles in the glass wool。4) S...
08-02-03摘要:Arachidonic Acid LabelingContributor: Suprya JayadevDate: November 4, 19911) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum。2) Pellet cells and wash 1 time with room t...
08-02-03摘要:AA Metabolite Quantitation of Media Post-AA Labeling Contributor: Suprya JayadevDate: November 4, 19941) Remove 2 500 µ。l aliquots of supernatant into scintillation vials, add scintillation ...
08-02-03摘要:AA Metabolite Quantitation of Cell Pellets Post-AA Labeling Contributor: Suprya JayadevDate: November 4, 1991Extraction:1) Following spin, save supernatant for analysis。2) Immediately upon removi...
08-02-03摘要:2 µ。2 µ。2 ml aqueous solution of purified porin or porin containing cell envelopes。 It is best to use a concentrated protein solution ( 1 mg/ml) so that most of the detergent in the pr...
08-02-03摘要:Choline Labeling and TNF Treatment of HL-60 CellsContributor: Suprya JayadevDate: Aug。 6, 19911) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum。2) Pellet cells and wa...
08-02-03摘要:Sphingomyelin Quantitation Post-choline Labeling of HL60 CellsContributor: Suprya JayadevDate: Jun。 @ 2 units/ml in 10 mM Tris-HCl (pH 7。5 ml into each of two duplicate glass pyrex tubes and main...
08-02-03摘要:2) Resuspend pellet in 3 ml CHCl3: CH3OH (1:2) and vortex hard。8 ml H2O, vortex hard and allow solution to sit at room temperature for atleast 30 minutes。6) Add 1 ml of CHCl3 and 1 ml of H2O to e...
08-02-03摘要:20, 19911) Pack a Bio-Sil A column as follows:a) Pack base of column with a small portion of glass wool。b) Suspend 20 grams of 100-200 mesh BioSil A in 100 ml chloroform。c) Pour silica/chloroform...
08-02-03摘要:Preparation of bOG-DOPG mixed micellesContributor: Date: November 22, 1993Materials:All glassware must be acid washed, rinsed thoroughly with water then rinsed with acetone and dried。Recrystallizi...
08-02-03