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Asymmetric sequences are indicated by *.
Single letter code:
R = G or A;
Y = C or T;
W = A or T;
M = A or C;
K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;
B = C, G or T;
D = A, G or T;
N = G, A, T or C.
Recognition
sequenceEnzyme AAT^ATT SspI AGC^GCT Eco47III AG^CT AluI AGG^CCT Eco147I AGT^ACT ScaI ATTT^AAAT SmiI CACGTC(-3/-3)^* BtrI CAC^GTG Eco72I CACNN^NNGTG OliI CAG^CTG PvuII CAYNN^NNRTG MslI CCC^GGG SmaI CCGCTC(-3/-3)^* MbiI CG^CG Bsh1236I CMG^CKG NspBII GAANN^NNTTC PdmI GACGTG(-3/-3)^* BtrI GACNN^NNGTC BoxI ^(5/5)GACTC* SchI GAGCGG(-3/-3)^* MbiI GAG^CTC Ecl136II GAGTC(5/5)^* SchI GAT^ATC Eco32I Gm6A^TC DpnI GATNN^NNATC BseJI GCCC^GGGC SrfI GCC^GGC PdiI GCN^NGC Cac8I GG^CC BsuRI GGC^GCC EheI GGN^NCC BspLI GT^AC RsaI GTA^TAC Bst1107I GTN^NAC Hpy8I GTY^RAC HincII GTT^AAC KspAI GTTT^AAAC MssI YAC^GTR BsaAI RG^CY CviJI RTGC^GCAY FspAI TAC^GTA Eco105I TCG^CGA Bsp68I TG^CA CviRI TGC^GCA NsbI TGG^CCA MlsI TTA^TAA PsiI TTT^AAA DraI