摘要：Mix 5 ml of the sample with 100 ml Salmon sperm DNA (50 mg in 20 mM EDTA)。 Add 5 mL ice cold 10% Trichloroacetic acid (TCA)。 Wash the filter 6 times with 5 mL ice-cold 10% TCA。 The second filter...

摘要：CIMMYT Applied Molecular Genetics Laboratory1DNA Quantification by SpotFluorescence1。 Spot several 3 &mu。l of a series of known DNA concentration (l DNA at 0, 1, 2。 Add 3 dilutions (1/2, 1/4, 1/...

摘要：Measurement of DNA concentration using PanVera fluorescence assaySteve HahnLast updated 9/30/98This Dye measures DNA concentration using a fluorescent dye which binds only double stranded DNA。 The...

摘要：UV Absorbance (280 nm) &ndash。 Protein Determination Simple and quick method to accurately quantitate total protein in purified material or approximately quantitate total protein in crude lysates ...

摘要：Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using Dot Blot method if you have both purified protein and specific antibody against...

摘要：Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups Author: David Van Horn, Greg Bulaj Source: Contributed by David Van Horn, Dept。 Peptides from these syntheses are usually in their...

摘要：Protein Assay (Spectrophotometer) Use BSA (bovine serum albumin) 1mg/ml stock solution (1ml Eppendorf tubes) for standard curve。 Place 0, 2, 5, 10, 15, 20µ。g (=µ。 Use 2-5µ。

摘要：Protein concentration of Laemmli gel samplesTo 10 µ。l boiled lysate (in Laemmli sample buffer) add 40µ。

摘要：Lowry Protein Assayreagents 2% Na2CO3 in 0。00 ml of 1 0。25 ml of 2 0。25 ml of 3 2N Folin reagent (yellow in color) Standard: Given BSA 1 mg/mlprocedure Transfer sample con...

摘要：Lowry &ndash。 Protein Determination(From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H。 Matthews) (Lowry, O。 (1951) Protein measu...

摘要：Phosphoamino acid analysis of non-TCA precipitable proteins1。 Elute phosphoamino acids with 2 x 0。

摘要：COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODS Company Method Detection Range Applications -Compatibility ...

摘要：BIURET PROTEIN ASSAYMATERIALS Biuret Reagent Bovine serum albumin (BSA) Spectrophotometer and tubesPROCEDURE Prepare standard dilutions of BSA containing 1, 2。5 and 10 mg/ml protein。0...

摘要：Biorad Protein Assay: Bradford Standards: 1 mg/ml BSA stock- dilute 1:10 to get 0。l concentrated Biorad reagent and incubate at room temperature for 5 minutes。3) Assay absorbance at 595 nm。

摘要：This list includes the sensitivity range, volume/amount of sample needed, subjective comments on accuracy and convenience, and major interfering agents。 The criteria for choice of a protein assay ...

摘要：Bradford &ndash。 Protein DeterminationIntroductionA rapid and accurate method for the estimation of protein concentration。 Bradford, M。 Reagents SolutionBovine Serum Albumine (BSA) 10X: 1mg/ml...

摘要：Bradford Protein Concentration Assayversion 01/07/2001Abbreviations: mcg = micrograms mcL = microliters BSA = bovine serum albumin O。 = optical density dI = deionizedBackgroundThe B...

摘要：Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay。 The Bradford is recommended for general use, especially fo...

摘要：Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration。278General Overview of Procedure: Dilute 5x Bradford Reagent (BioRad) 4:1 with wat...

摘要：Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample。 ReferenceBradford, M。

摘要：Quantitation of DNAFluorescent quantitation Wear gloves and goggles protecting from EtBr and UV light With an EDP (dilute mode) pick up 9 µ。l vortexed DNA sample。l of these dilution on diffe...

摘要：Characterization of dnaLEVEL I Materials DNA sampleSSC bufferUV spectrophotometer 3 and quartz cuvettes Procedure Dissolve a small quantity of your extracted DNA in 3。 Turn on and blank a UV spect...

摘要：Plate assay for determination of DNA concentrationA fairly accurate, rapid assay of DNA concentration can be obtained by UV visualization of samples spotted onto ethidium bromide-containing agarose...

摘要：Proteins are measured (directly) at 280 nm, nucleic acids at 260 nm and colorimetric protein determination is carried out in the range from 550 to 600 nm。 The concentration is determined by measur...

摘要：This program helps you to calculate the concentration of nucleic acids according to their optical density。 (DNA, RNA, oligonucleotides)。 It is better to use {OD260-OD320} instead of OD260。 For a...

摘要：Put 5m l of DNA concentration。 Repeat procedure 3 times with new samples of DNA noting readings at both A260 and A280。 Take an average of the A260 readings and multiply the figure by 10 to give t...

摘要：Steve HahnLast updated 9/30/98This Dye measures DNA concentration using a fluorescent dye which binds only double stranded DNA。 The fluorescence of this dye is greatly amplified when bound to DNA。...

摘要：Proteins are measured (directly) at 280 nm, nucleic acids at 260 nm and colorimetric protein determination is carried out in the range from 550 to 600 nm。 The concentration is determined by measur...