高效液相色谱法测定密蒙花中蒙花苷的含量

摘　要：目的：建立高效液相色谱法测定密蒙花中蒙花苷的含量。方法：采用RP-C18色谱柱，甲醇-水-醋酸(45:54.5:0.5)为流动相，流速0.8mL·min^-1,326nm为检测波长，室温下对密蒙花中的蒙花苷进行含量测定。结果：蒙花苷在0.4～2.9μg范围内.面积与其浓度线性关系良好(r=0.9991)；样品平均回收率为97.02％,RSD为1.5％(n=4)。结论：该法简单、快速、结果准确可靠。

关键词：高效液相色谱法，密蒙花，蒙花苷

分类号：R927　文献标识码：A

Determination of Linarin in Buddleja officinalis Maxim. By RP- HPLC

WANG Jun - xian（College of Pharmacy, Xi＇an Jiaotong University, Xi＇an 710061）　
LI Jiao - she（College of Pharmacy, Xi＇an Jiaotong University, Xi＇an 710061）　
YANG Guang - de（College of Pharmacy, Xi＇an Jiaotong University, Xi＇an 710061）　
LU Ping（College of Pharmacy, Xi＇an Jiaotong University, Xi＇an 710061）　
ZHU Yu - hong（College of Pharmacy, Xi＇an Jiaotong University, Xi＇an 710061）　

Abstract：Objective: To establish a method using RP - HPLC for determination of the content of linarin in Buddleja officinalis Maxim. Methods: RP- C18 column was used, the mobile phase was methanol - water- acetic acid (45:54.5: 0.5 ) at a flow rate of 0.8 Ml·min^-1 , the UV detection wavelength was 326 nm and column temperature was room temperature. Results: The calibration curve was linear ( r = 0. 999 1 ) in the range of 0.4 ～ 2.9 μg for linarin, the mean recovery was 97.02 %, with RSD 1.5 % ( n = 4). Conclusion: This method is simple, rapid and reliable.

Keywords：HPLC, Buddleja officinalis Maxim., linarin

参考文献：

［1］ChP(中国药典).2000. Vol I(一部)：269
［2］LI Xiu - lan (李秀兰)，SUN Guang - jie (孙光洁),DAI Shu pei (戴树培)，et al. Bacteriostasis of main ingredients in Flos Buddlejae and Edgeworthia hrysantha (密蒙花/结香有效成分的抑菌作用).Northwest Pharm (西北药学杂志)，1996,11(4):165 (in Chinese)
［3］LI Jiao - she (李教社)，ZHAO Yu - ying (赵玉英)，WU Bin (王?)，et al. Separation and identification of the fiavonoids from Buddleja officinalis Maxim (密蒙花黄酮类化合物的分离与鉴定).Acta Pharm Sin(药学学报)，1996,31(11):849(in Chinese)

收稿日期：2001年1月17日

出版日期：2001年3月31日