HPLC法测定白花前胡中白花前胡丙素和紫花前胡中紫花前胡苷的含量?

摘　要：目的：建立高效液相色谱法测定白花前胡根中白花前胡丙素(dl-praeruptorinA,Pd-Ia)和紫花前胡根中紫花前胡苷(nodakenin,NDK)含量的方法。方法：色谱柱Shim-PackCLC-ODS,Pd-Ia的流动相为甲醇-水(79∶21)和NDK的流动相为乙腈-甲醇-水(20∶5∶75），流速为1.0m^.Lmin^-1，紫外检测波长分别为323和334nm。结果：Pd-Ia及NDK的线性范围分别为0.08～0.8μg和0.01～0.10μg，相关系数均为0.9999，平均回收率分别为98.53%～99.27%和97.60%～99.87%,RSD小于2%。结论：方法准确，简便，快速，灵敏度高，对常用中药前胡的质量控制有较重要意义。

关键词：白花前胡　紫花前胡　白花前胡丙素　紫花前胡苷　高效液相色谱

分类号：R927　文献标识码：A

HPLC Determination of dl-Praeruptorin A in Peucedanum praeruptorum Maxim.and Nodakenin in Peucedanum decursivum Dunn.

XU Qin（Department of Pharmaceutical Analysis,Guilin Medical College,Guilin 541004）　
LIU Bu-ming（Guangxi Institute of Traditional Chinese Medicine,Nanning 530022）　
ZHANG Zheng-xing（Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 210009）　

Abstract：Objective：To establish a method for determination of the contents of dl-praeruptorin A(Pd-Ia)in roots of Peucedanum praeruptorum Maxim.and nodakenin(NDK) in roots of Peucedanum decursivum Dunn.by HPLC.Methods:The chromatographic condition:Shim-Pack CLC-ODS column(6.0 mm×150 mm,10 μm),CH₃OH-H₂O(79∶21)for Pd-Ia and H₂O-CH₃OH-CH₃CN(75∶5∶20)for NDK as mobile phase,the flow rate was 1.0 mL^.min^-1,the column temperature was 40 ℃,the detection wavelength was 323 nm,334 nm for Pd-Ia and for NDK.The calibration curves showed good linearity in the range of 0.08～0．8　μg and 0.01～0.1 μg,r=0.999 9 and 0.999 9 respectively.The average recoveries were 98.53%～99.27% and 97.60%～99.87%,RSD was less than 2% respectively.Conclusion:The method is simple,sensitive,rapid and accurate,and can be used for the quality control of Chinese medicine Qianhu.

Keywords：Peucedanum praeruptorum Dunn,Peucedanum decursivum Maxim,dl-praeruptorin A(Pd-Ia),nodakenin(NDK),HPLC

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收稿日期：2000年1月10日

出版日期：2001年3月31日