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摘 要：建立了一个快速、简单、准确的固相萃取和高效液相色谱相结合的测定苦瓜甙A含量的方法。样品经石墨碳固相萃取管（3mL/250mg）纯化后以高效液相色谱检测。色谱柱为C18，流动相为V(乙腈)∶V(甲醇)∶V(50mmol/L磷酸二氢钾缓冲液)=25∶20∶60，流速为0.8mL/min，检测波长为208nm。标准曲线自10mg/L到1000mg/L呈线形关系（r2=0.9992）。该方法具有很好的重现性，日内或日间的相对标准偏差和相对平均误差均小于10%。样品回收率大于90%。
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Abstract：A
rapid, simple and accurate method for the determination of momordicoside
A has been established using solid phase extraction (SPE) followed by
high performance liquid chromatography (HPLC). Sample was processed by
SPE on a Carb cartridge (3 mL/250 mg), and was then determined by HPLC
on a C18 column (4.6 mm i.d.×250 mm, 5 μm)
with V(acetonitrile)∶V(methanol)∶V(50
mmol/L potassium dihydrogen phosphate buffer)=25∶20∶60
as mobile phase (0.8 mL/min) and UV detection at 208 nm. The calibration
curves were linear from 10 mg/L to 1000 mg/L (r2=0.9992). The
analytical method was shown to be highly reproducible, giving all of the
relative standard deviations and relative mean errors less than 10% for
both intra-day and inter-day determinations. The absolute recoveries
were greater than 90%. |
| 基金项目：This work
was supported by the key research program of Education Ministry of China 作者简介：WANG Shui (1965-), male, master, engineer, Tel: (028) 5411958. 作者单位：王水（四川大学生命科学学院，四川成都 610064） 唐琳（四川大学生命科学学院，四川成都 610064） 郭亦然（四川大学生命科学学院，四川成都 610064） 颜钫（四川大学生命科学学院，四川成都 610064） 陈放（四川大学生命科学学院，四川成都 610064） 参考文献： ［1］Ho W K K, Liu S C, Shaw P C, et al. Biochem
Biophys Acta, 1991, 1088: 311-314 |
原载于《色谱》2001 Vol.19 No.2
P.128-131
http://periodical.wanfangdata.com.cn/periodical/sp/sp2001/0101/0101ml.htm