摘要:将罐头食品捣碎后用水稀释,加氯化铜溶液和抗坏血酸,用水定容,过0.45 μm滤膜后立即供液相色谱分析,在254 nm波长处测定。色谱柱:Hypersil ODS(125 mm×4 mm i.d., 5 μm);流动相:水-甲醇(体积比为80∶20),含有20 mmol/L四丁基溴化铵,0.03 mol/L 醋酸钠缓冲液(pH 4) ,流速:0.8 mL/min;进样量:20 μL。EDTA在10 mg/L~400 mg/L范围内呈线性。方法回收率为95.5%~98.9%,其RSD为0.82%~1.32%。
Abstract:The homogenized sample is diluted with water. After adding copper(Ⅱ) chloride solution and 20 mg of ascorbic acid, dilute the sample to the volume with water. After thorough mixing and microfiltration over a 0.45 μm filter,the filtrate is ready for injection into the HPLC system and detected at 254 nm.The 125 mm×4 mm i.d. chromatographic column is packed with Hypersil ODS 5 μm,eluted with a mobile phase of water-methanol (80∶20,V/V) containing 20 mmol/L tetrabutyl ammonium bromide, 0.03 mol/L sodium acetate buffer (pH 4) at a flow rate of 0.8 mL/min. The injection volume is 20 μL. The response value was linear between 10 mg/L-400 mg/L. The recovery was 95.5%-98.9%. The RSD was 0.82%-1.32%.
作者简介:施旭霞(1963-),女,工程师。
通讯联系人:陈笑梅(1965-),女,高级工程师,电话:(0571)8381111-62004。
施旭霞(浙江进
出口商品
检验局,浙江 杭州 310012)
陈笑梅(浙江进出口商品检验局,浙江 杭州 310012)
参考文献:
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[2] St[lberg Olle,Arvidsson Torbjirn. J Chromatogr A,1994, 684: 213-219
[3] Mika Sillanpää,Raimo Kokkonen,Marja-Liisa Sihvonen. Analytica Chimica Acta, 1995,303:187-192
原载于《色谱》2000 Vol.18 No.5 P.445-447
http://www.chinainfo.gov.cn/periodical/sp/sp2000/0005/0005ml.htm
作者:
2007-5-18