摘要：采用高效液相色谱法同时测定喘静片中的3种主要成分：茶碱、异戊巴比妥和盐酸甲基麻黄碱。以ODS作固定相，通过均匀设计方法选择的最佳流动相为V（0.015 mol/L磷酸二氢钠缓冲液，含体积分数为0.3%的三乙胺，pH 4.9 )∶V（甲醇）=35∶65，检测波长为215 nm，用程序调节检测器的灵敏度，以咖啡因作内标物。茶碱、异戊巴比妥、盐酸甲基麻黄碱的回收率分别为99.7%～102.6%，98.5%～100.2%，98.0%～102.7%，日间RSD分别为0.23%～1.2%，0.35%～2.5%，0.33%～1.6%。该方法简便、快速、准确。
Abstract:Uniform design method was employed to optimize the mobile phase of HPLC in order to determine simultaneously three principal components, theophylline, amobarbital and methylephedrine hydrochloride, in Chuanjing tablet, a compound preparation for asthma and cough. The stationary phase was ODS and the optimal mobile phase composition was V(0.015 mol/L phosphate buffer containning 0.3% triethylamine, pH 4.9)∶V(methanol)=35∶65. The detection was performed at 215 nm and the sensitivity was time programmed for simultaneous determination of minor and major components. Caffeine was selected as the internal standard. A baseline separation was achieved within 10 min. The linear ranges for theophylline, amobarbital and methylephedrine hydrochloride were 0.03 g/L-0.20 g/L, 7.5 mg/L-50.0 mg/L and 7.5 mg/L-50.0 mg/L, recoveries 99.7%-102.6%, 98.5%-100.2% and 98.0%-102.7%, inter-day RSDs 0.23%-1.2%, 0.35%-2.5%,0.33%-1.6% respectively. This HPLC method is rapid and accurate, and suitable for the quality control of the preparation.
作者简介：李发美（1947-），女，博士，教授，电话：（024）23843711，传真：（024）83890024，E-mail:fameli@ihw.com.cn。
李发美（沈阳药科大学分析化学教研室， 辽宁 沈阳 110015）
邱枫（沈阳药科大学分析化学教研室， 辽宁 沈阳 110015）
王伟红（沈阳药科大学分析化学教研室， 辽宁 沈阳 110015）
郭兴杰（沈阳药科大学分析化学教研室， 辽宁 沈阳 110015）
邸欣（沈阳药科大学分析化学教研室， 辽宁 沈阳 110015）
参考文献：
［1］ Liaoning Public Health Bureau(辽宁省卫生厅). Liaoning drug standard(辽宁省药品标准). Shenyang(沈阳)：［s.n.］(［出版者不详］),1987.801
［2］ Zhang J, Tian Z, Lou Z C. Planta Medica, 1988,54(1):69-70
［3］ LIU Shu-chun(刘树春). Chinese Journal of Pharmaceutical Analysis(药物分析杂志)，1991,11(1):7-9
［4］ Sun P,Mariano G,Barker G.Anal Lett,1994,27(5):927-937
［5］ Papadoyannis I, Georgarakis M, Samanidou V, et al. J Liq Chromatogr, 1991,14(8):1 587-1 603
［6］ HU Jia-chi,WANG Zu-fa,WENG Wen-hui(胡家炽，王祖法，翁文辉). Chinese Journal of Pharmaceutical Analysis(药物分析杂志)，1990,10(2):97-99
［7］ ZHUO Hai-tong,LIU Chao(卓海通，刘 超). Chinese Journal of Chromatography(色谱)，1991,9(1):49-52
［8］ WANG Xiao-qin,DENG Bo,QIN Jian-hou(王小芹，邓勃，秦建侯). Chinese Journal of Analysis Laboratory(分析试验室)，1985,4(12):46-54
原载于《色谱》2000 Vol.18 No.5 P.442-444
http://www.chinainfo.gov.cn/periodical/sp/sp2000/0005/0005ml.htm
作者：
2007-5-18