摘要:建立了用超滤-毛细管电泳技术测定血清中唾液酸含量的方法, 并考察其最佳操作条件。 该方法分离分析唾液酸可直接用紫外检测(195 nm),不需要柱前、柱后衍生。方法的回收率为92.6%,最低检测浓度为9.6 μmol/L,最低检测量为39 fmol。用该法测定了30例正常人和11例
肺癌患者血清中唾液酸的含量,结果表明后者比前者有显著性增加(P<0.001)。 用该方法测定的结果与传统使用的比色法结果之间呈良好的线性关系(r= 0.983,n=10) 。方法简单、灵敏,适合恶性
肿瘤防治的基础研究和
临床应用。
Abstract:A method for the determination of sialic acids in serum with ultrafiltration-capillary electrophoresis is described and the operation was optimized. Sialic acids were directly separated and analyzed with UV detection at 195 nm and without pre-or post-column derivatization. The recovery of N-acetylneuraminic acid (NANA) was 92.6%, the concentration and mass detection limit of NANA were 9.6 μmol/L and 39 fmol respectively. This method was used for the determination of NANA level in the serum of 11 lung cancer patients and 30 normal adults. The results showed that the average concentration of NANA in the serum of patients was much higher than that of normal adults with P<0.001. The results were also compared with those obtained by the traditional colorimetric method, with good linear relationship of r=0.983 at n=10. It is concluded that the method described in this paper is simple and sensitive, and is suitable for basic research and clinical applications to malignant tumors.
基金项目:国家自然科学基金资助项目(29635020)
作者简介:董秀玲(1961-),女,副教授,工作单位:大连市第三人民医院,现为大连化学物理研究所在读博士研究生。
通讯联系人:林炳承,博士生导师,研究员,电话:(0411)4671991-860,传真:(0411)3622302。
董秀玲(中国科学院大连化学物理研究所, 辽宁 大连 116023 大连市第三人民医院, 辽宁 大连 116033)
戴忠鹏(中国科学院大连化学物理研究所, 辽宁 大连 116023)
林炳承(中国科学院大连化学物理研究所, 辽宁 大连 116023)
鞠秀君(大连市第三人民医院, 辽宁 大连 116033)
王晓伟(大连市第三人民医院, 辽宁 大连 116033)
袁小林(大连市第三人民医院, 辽宁 大连 116033)
参考文献:
[1] Schauer R.Trends Biochem Sci,1985,10:357-360
[2] Stringou E, Chondros K, Kouvaris J, et al. Anticancer Res,1992,12:251-255
[3] Crook M.Clin Biochem, 1993,26:31-38
[4] Kobayashi K, Akiyama Y, Kawaguchi K, et al. Anal Sci, 1985,1:81-84
[5] Hara S, Yamaguchi M, Takemori Y, et al. J Chromatogr, 1986,377:111-119
[6] Karamanos N K,Wikstrim B,Antonopoulos C,et al.J Chromatogr,1990,503:421-429
[7] Zhou G,Yu Q N,Ma Y F,et al.J Chromatogr A,1995,717:345-349
[8] Han F T,Huynh B H,Shi H L,et al.Anal Chem,1999,71:1 265-1 269
[9] Makatsori E,Fermani K,Aletras A,et al.J Chromatogr, 1998,712:23-29
[10] Kakari S,Stringou E,Toumbis M,et al.Anticancer Res,1991,11:2 107-2 110
原载于《色谱》2000 Vol.18 No.5 P.426-428
http://www.chinainfo.gov.cn/periodical/sp/sp2000/0005/0005ml.htm
作者:
2007-5-18