摘要：采用简便易行的方法自制内径为100 μm的电色谱柱,并考察了其性能,同时研究了磷酸盐缓冲液浓度、乙腈体积分数、电压、柱温对中性化合物保留行为的影响。
Abstract:The packed column was prepared with a simple method by combination of vacuum filling and pressure compressing. The reproducibility of efficiency and retention time was evaluated. The mixture of thiourea and three aromatic compounds was used. RSD values of retention time were smaller than 2.2% and RSD values of efficiency were smaller than 7.5%. The influence of voltage, phosphate concentration,acetonitrile volume fraction and column temperature on the electroosmotic flow and capacity factor of neutral compounds was investigated. Acetonitrile concentration had significant effect on the electroosmotic flow and capacity factor of neutral compounds. High concentration of acetonitrile improved the elution ability. Low concentration of acetonitrile improved the resolution. Voltage,phosphate concentration,and column temperature also had effects on the electroosmotic flow and capacity factor of neutral compounds,but the effects were smaller than the concentration of acetonitrile. These results gave a guide to select separation conditions in capillary reversed-phase electrochromatography.
资助项目：本项目由分析测试协会资助，项目批准号为9703-62
作者简介：张金兰(1969-)，女，博士研究生，助研，电话：(010)63165236。
张金兰(中国协和医科大学 中国医学科学院药物研究所分析室, 北京 100050)
周同惠(中国协和医科大学 中国医学科学院药物研究所分析室, 北京 100050)
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原载于《色谱》2000 Vol.18 No.5 P.420-422
http://www.chinainfo.gov.cn/periodical/sp/sp2000/0005/0005ml.htm
作者：
2007-5-18