摘要：建立了大鼠神经组织中亚硝酸盐和硝酸盐的高效液相色谱测定方法，以用于研究一氧化氮在
糖尿病慢性神经病变中的作用。在1 μmol/L～25 μmol/L的浓度范围内，NO2-和NO3-的峰面积与浓度的线性相关系数>0.991；最低检测浓度分别为0.2 μmol/L和0.5 μmol/L；日内、日间相对标准偏差<14%。对各实验组大鼠的初步测定结果表明，糖尿病组及糖尿病胰岛素(IGF)治疗组的NO2-和NO3-水平均低于对照组。
Abstract:To study the effect of nitric oxide on the diabetic neuropathy, a quantitative method for the determination of nitrite(NO2-) and nitrate(NO3-) in rat nervi ischiadicus by high performance liquid chromatography(HPLC) with UV-VIS detection was developed. In the range of 1 μmol/L-25 μmol/L, correlation coefficient between concentration and peak area for NO2- and NO3- was more than 0.991. The minimum detection limits for both ions were 0.2 μmol/L and 0.5 μmol/L, respectively. Relative standard deviation（RSD）of inter-day and intra-day was less than 14%. The effects of different acetonitrile concentration and pH of mobile phase on separation as well as the stability of both salty ions were studied. In comparison with normal group , the levels of NO2- and NO3- for diabetic groups were lower. Measured mean values were, in turn, as follows: normal rats,(2.62±0.38) μmol/L and (24.43±5.61) μmol/L (n=8); diabetic-controlled rats(saline-given only),(2.24±0.20) μmol/L and (17.15±7.79) μmol/L (n=6); diabetic rats treated with bovine colostrum insulin-like growth factor(BctIGF-1) or with recombinant human insulin-like growth factor(rhIGF-1),(2.55±0.36) μmol/L and (6.12±2.14) μmol/L (n=5) and (1.46±0.08) μmol/L and (9.95±3.34) μmol/L (n=7), respectively.
作者简介：朱秋毓(1952-)，男，主管技师，电话：(021)62489999-6458。
朱秋毓(华山医院糖尿病研究室，上海 200040)
周一鸣(华山医院糖尿病研究室，上海 200040)
俞茂华(华山医院糖尿病研究室，上海 200040)
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原载于《色谱》2000 Vol.18 No.5 P.387-389
http://www.chinainfo.gov.cn/periodical/sp/sp2000/0005/0005ml.htm
作者：
2007-5-18