摘 要 首次报道了应用聚二甲基硅氧烷纤维作为固相微萃取的萃取头，色-质联用法快速测定海水中角鲨烯的方法。研究了萃取平衡时间、搅拌速度、介质离子强度、pH值、以及解吸温度、解吸时间等条件对测定效果的影响。该方法具有简便、快速、灵敏度高、不使用有毒有机溶剂的优点，其检测限为0.005 μg/L，相对标偏差介于4.9%～6.2%，回收率在92.0%～95.0%之间，能有效地应用于海水中角鲨烯的测定。海水中角鲨烯浓度的变化，可能作为赤潮预报的一个指标，通过对其变化的监测，为海洋赤潮的预报、防治提供科学依据。
Abstract A method for the determination of squalene in seawater was developed by using solid-phase microextration with 100 μm polydimethylsiloxane coating and gas chromatography-mass spectrometry. The effects of balance time, stirring speed and ionic strength of medium on extraction efficiency of squalene were investigated. The method has the advantages of simple operation, rapid, high sensitivity and without using toxic solvent. The limit of detection is 0.005 μg/L, the relative standard deviation ranged from 4.2% to 6.2%, and the recovery ranged from 92.0% to 95.0%. It has been applied for the determination of squlaene in seawater. It was found that the concentration of squlaene in non-redtide seawater was 42% higher than that in redtide seawater. Squalene in seawater may be used as a marker of marine redtide. Monitoring the variation of squalene concentration in seawater may provide a scientific basis for forecasting marine redtide.
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自《分析化学》2000-11期，
全文：http://www.chinainfo.gov.cn/periodical/fxhx/fxhx2000/0011/0011ml.htm
作者：
2007-5-18