血透患者上机前静脉管路端液体中微量戊二醛的检测

摘　要：目的　建立用HPLC测定血透患者上机前静脉端管路流出液体中微量戊二醛浓度的方法，为临床进一步研究微量戊二醛的来源以及微量戊二醛的清除提供实验依据。方法　取流出液体20mL,与二硝基苯肼柱前衍生化3h,过滤，进样20μL。色谱条件：色谱柱：C₁₈柱；柱温：29℃；流动相：乙腈-磷酸（60∶40）；流速：1mL^.min^-1；检测波长：365nm。结果　血透患者上机前静脉端管路流出液体中存在有微量的戊二醛。结论　HPLC方法能简便，准确地测出血透上机前静脉端管路流出液体中微量戊二醛。

关键词：高效液相色谱法；戊二醛；血透

分类号：R917；R927.2　文献标识码：A
文章编号：10011-2494(2001)01-0057-03

Detection of trace glutaraldehyde in the fluid from venous circuit tube by HPLC

LIU Fei-fan（Wuhan General Hospital,Wu Han 430070，China）　
LI Xiao-ping（Wuhan General Hospital,Wu Han 430070，China）　
ZHANG Li-hua（Wuhan General Hospital,Wu Han 430070，China）　
LAN Tian-biao（Wuhan General Hospital,Wu Han 430070，China）　
GUO Si-heng（Wuhan General Hospital,Wu Han 430070，China）　
WANG Ying-qun（Wuhan General Hospital,Wu Han 430070，China）　

Abstract：OBJECTIVE　To detect the trace glutaraldehyde in the fluid from venous circuit tube of hemodialysis,a HPLC assay was developed.METHODS　20 mL fluid taken from the venous circuit tube were derived with DNPH for 3 h,then filtered and injected. Chromatography was conducted on C₁₈ column at 29℃.The mobile phase was consisted of 60% CH3N and 40% H₃PO₄ with the flow rate at 1 mL^.min^-1, and the detetion wavelength was at 365 nm.RESULTS　The concentrations of glutaraldehyde in the fluid ranged from 14.99 to 37.40 μg^.mL^-1.CONCLUSION　This HPLC method is simple and accurate to detect the trace glutaraldehyde remained in the fluid from venous circuit tube.

Keywords：HPLC；glutaraldehyde；hemodialysis

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收稿日期：1999年6月28日

出版日期：2001年1月8日