乳结消散片中丹参酮ⅡA的含量测定

摘　要：目的　建立高效液相色谱法测定丹参酮ⅡA含量的方法。方法　C₁₈柱以甲醇-水（70∶30）作为流动相，检测波长269nm。结果　丹参酮ⅡA浓度在0～33ng^.mL^-1范围内，峰面积与进样量线形关系良好，回归方程Y=9725X-2584，r=0.9997。平均回收率为98.29%，RSD=1.86%。结论　本法重现性好，准确快速，可作为质量控制指标。

关键词：丹参酮ⅡA；高效液相色谱法；含量测定

分类号：R917；R927.2　文献标识码：A
文章编号：1001-2494(2001)01-0056-02

Determination of tanshinoneⅡA in Rujie Xiaosan tablet

YANG Yi-heng（Department of Pharmacy,The third school of Clinical medicine，Beijing University,Beijing 100083，China）　
ZHANG Xiao-le（Department of Pharmacy,The third school of Clinical medicine，Beijing University,Beijing 100083，China）　
WANG Shu-qing（Department of Pharmacy,The third school of Clinical medicine，Beijing University,Beijing 100083，China）　
YAN Bao-xia（Department of Pharmacy,The third school of Clinical medicine，Beijing University,Beijing 100083，China）　

Abstract：OBJECTIVE　To develope an HPLC assay for the determination of tanshinoneⅡA.METHOD　C₁₈ODS column was used.The mobile phase was consisted of MeOH-water(70∶30).The detection wavelength was at 269 nm.RESULT　The linear regression equation was Y=9725X-2584,r=0.9997.The average recovery was 98.29% with RSD=1.86%.(n=5).CONCLUSION　The method may be used for quality control.

Keywords：tanshinoneⅡA；HPLC；determination

参考文献：

［1］　谭生建，陈培让，王建社，等．RP-HPLC法测定抗栓保心片中丹参酮ⅡA的含量[J].中国中药杂志,1999,24(3):154.
［2］　 蒋学华,许启荣,罗娟，等.高效液相色谱法测定复方丹参片中丹参酮ⅡA的含量[J].华西药学杂志,1996,11(2):102.
［3］　伍丕娥,刘仲义. 高效液相色谱法测定肝福冲剂中丹参酮ⅡA的含量[J].中成药,1995,17(6):13.

收稿日期：1999年12月24日

出版日期：2001年1月8日