高效液相色谱法测定气喘片中4种成分的含量

摘　要：目的　采用反相高效液相色谱法同时测定气喘片中氨茶碱、盐酸麻黄碱、苯巴比妥和盐酸苯海拉明的含量。方法　用LichrosorbC18为色谱柱填料，以0.2%的三乙胺溶液(用50%的磷酸调节pH值2.5)-甲醇(50∶50)为流动相，检测波长254nm。结果　4种成分能很好分离；氨茶碱、盐酸麻黄碱、苯巴比妥和盐酸苯海拉明的线性范围分别为20～300μg·mL^-1(r=0.9991),5～75μg·mL^-1(r=0.9999),10～150μg·mL^-1(r=0.9999)和5～75μg·mL^-1(r=0.9997)；平均回收率分别为101.18%，97.09%，100.50%和100.78%，RSD＜2%(n=5)。结论　方法快速、方便，适用于该复方制剂中4种成分的同时测定。

关键词：高效液相色谱法；气喘片；氨茶碱；盐酸麻黄碱；苯巴比妥；盐酸苯海拉明

分类号：R917.1　文献标识码：A
文章编号：1001-2494(2001)03-0189-03

Determination of four constituents of antiasthma tablets by HPLC

LU Guo-hong（Department of Pharmacy,Renji Hospital,Shanghai Second Medical University,Shanghai 200001,China）　
WANG Ping-quan（Department of Pharmacy,Renji Hospital,Shanghai Second Medical University,Shanghai 200001,Chinac）　

Abstract：OBJECTIVE　To establish a method for the determinatrion of the four constituents in antiasthma tablets by RP-HPLC:aminophylline,ephedrine hydrochloride,phenobarbital and diphenhydramine hydrochloride.METHODS　Lichrosorb C18 column was used.The mobile phase was 0.2% triethylamine adjusted to pH 2.5 with 50% phosphoric acid-methanol(50∶50).The UV detection wavelength was 254 nm.RESULTS　The four constituents had good resolution.The liner range of aminophylline,eyhedrine hydrochloride,phenobarbital and diphenhydramin hydrochloride were 20～300 μg·mL^-1,5～75 μg·mL^-1,10～150 μg·mL^-1 and 5～75 μg·mL^-1 with the correlation coefficients 0.9991,0.9999,0.9999 and 0.9997,respectively.The average recoveries were 101.18%,97.09%,100.50% and 100.78% respectively with RSD＜2%(n=5).CONCLUSION　This method was simple and fast,and suitable for the determination of the four constituents in compound tablets.

Keywords：HPLC;antiasthma tablet;aminophylline;eyhedrine hydrochloride;phenobarbital;diphenhydramine hydrochloride

参考文献：

［1］　　陈希贤，吕建新，李东.高效液相色谱分法同时测定人血清中茶碱与苯巴比妥、苯妥英、卡马西平的含量［J］.中国医院药学杂志，1994，14(9)：389.
［2］　　李世彩，刘泳.喘宁片二组分(巴比妥、盐酸麻黄碱)的高效液相色谱测定［J］.中国医药工业杂志，1995，26(12)：539.
［3］　　张家美，范志明，许克宁.HPLC法测定霜剂中盐酸利多卡因与苯海拉明的含量［J］.药学进展，1994，18(3)：153.

收稿日期：2000年9月27日

出版日期：2001年3月1日