3种灵芝多糖的研究

摘　要：目的　对3种灵芝多糖的含量、单糖组成和糖蛋白含量进行研究。方法　提取和纯化灵芝子实体、菌丝体和发酵液多糖，应用高效液相色谱法和凯氏定氮法对多糖的含量、单糖组成及糖蛋白质含量作了分析、比较。结果　3种灵芝多糖含量分别为：子实体含0.75%；菌丝体含2.13%；发酵液含0.982g^.L^-1。3种多糖均由葡萄糖、果糖组成，这两种单糖在子实体和菌丝体多糖中的摩尔比相同，为7∶1；在发酵液多糖中的摩尔比为2∶1。3种灵芝多糖中糖蛋白含量分别为：子实体多糖含12.22%；菌丝体多糖含26.62%；发酵液多糖含2.92%。结论　研究结果为综合开发利用灵芝提供了科学依据。

关键词：灵芝；多糖

分类号：R284.2　文献标识码：A
文章编号：1001-2494(2001)04-0237-03

Studies on three kinds of polysaccharide of Ganoderma lucidum

CHEN Xiao-mei（Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100094,China）　
GUO Shun-xing（Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100094,China）　
GAO Qiang（Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100094,China）　
XIAO Sheng-yuan（Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100094,China）　
YANG Jun-shan（Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100094,China）　

Abstract：OBJECTIVE　To study the polysaccharide in fruit-body,mycelia and fermenting liquor of Ganoderma lucidum.METHODS　Polysaccharide of G.lucidum were extracted and purified.The content,molar ratio and protein content of polysaccharide were determined by HPLC and Kjeldahl method.RESULTS　All polysaccharides consisted of glucose and fructose.The sugar components were 0.75% in fruit-body,2.13% in mycelia and 0.982 g^.L^-1 in fermenting liquor respectively.Their molar ratio was 7∶1 in fruit-body and mycelia and 2∶1 in fermenting liquor respectively.Their protein contents were 12.22%,26.62% and 2.92% in fruit-body,mycelia and fermenting liquor respectively.CONCLUSION　The experimental results supply the important scientific data for developing Ganoderma lucidum in medicinal field.
Keywords：Ganoderma lucidum;polysaccharide

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收稿日期：2000年9月21日

出版日期：2001年4月8日