毛细管电泳中葡萄糖-羟丙基甲基纤维素体系分离脱氧核糖核酸

摘　要：葡萄糖作为羟丙基甲基纤维素(Hydroxypropylmethylcellulose,HPMC)筛分体系的添加剂，可以改善该体系在低浓度时分离脱氧核糖核酸(DNA)的能力。研究了硼酸浓度和pH值对葡萄糖-羟丙基甲基纤维素体系分离性能的影响；并将葡萄糖与其它添加剂如甘油、甘露醇对分离的影响作了比较，葡萄糖特有的环状结构使得其对羟丙基甲基纤维素体系分离能力的提高更为显著。

关键词：毛细管电泳，脱氧核糖核酸，葡萄糖

分类号：O65　文献标识码：A

Separation of Deoxyribonucleic Acid in Glucose-Hydroxy-Propylmethyl Cellulose Solution by Capillary Electrophoresis

Jin Yan（Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023）　
Lin Bingcheng（Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023）　
Fung Yingsing（Department of Chemistry,The University of Hong Kong,Hong Kong）　

Abstract：Glycerol,mannitol has been used as additives for the separation of deoxyribonucleic acid (DNA)fragments in entangled solution by capillary electrophoresis. Differing from glycerol and mannitol,the structure of glucose is a ring with 5 hydroxyl groups.This structure helps it easily to form complex with boric acid and hydroxypropyl methylcellulose (HPMC).On the other hand,glucose has large space obstacle compared with glycerol and mannitol.So glucose can markedly improve separation of DNA in HPMC solution by capillary electrophoresis compared with other additives.The factors such as boric acid concentration and pH,influencing formation of complex of HPMC and glucose were studied. High concentration of boric acid could improve the DNA separation in glucose-HPMC matrix system. The optimization pH to separate DNA in HPMC-glucose solution by capillary electrophoresis was 8.3.
Keywords：Capillary electrophoresis,deoxyribonucleic acid fragment, glucose

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收稿日期：2000年6月7日

修稿日期：2000年10月10日

出版日期：2001年5月20日