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摘 要:建立了尿中三唑仑的代谢物α-羟基三唑仑和阿普唑-仑的代谢物α-羟基阿普唑仑的气相色谱?电子捕获检测法。代谢物用N,O-双三甲基硅烷基-三氟乙酰胺(BSTFA)进行三甲基硅烷衍生化后进行检测。尿中代谢物的检出限约为1μg/L(S/N=3),至少可检出口服治疗量药物人体24h之内排泄尿中的代谢物,满足麻醉后犯罪案件中药物检验的要求。
Determination of α-Hydroxytriazolam and α-Hydroxyalprazolam in Human Urine by Gas Chromatogrphy/Electron-Capture Detector Jiang Zhaolin(Department
of Forensic Medicine, Criminal Police College of China, Shenyang 110035) Abstract:A
method was developed to assay α-Hydroxyltriazolam
and α-Hydroxyalprazolam, which
are the major metabolites of triazolam and alprazolam respectively,in
human urine. After addition of 2-hydroxyflurazepam (interal
standard) and hydrolysis with β-glucuronidase,
the hydroxy-metabolites were extracted with hexane-dichloromethane (1∶1)
at pH 10.8, then were derivated with (BSTFA). The analysis was performed
on a HP-5 capillary column with electron-capture detector.The detection
limits of analysis in urine were about 1μg/L.The
method was successfully applied to urine specimens collected from
healthy human volunteers who ingested 0.5 mg of triazolam or 0.8 mg
alprazolam. The method was enough sensitive to assay urine specimen
excreted at 24 h by volunteers after taking the medicine. |
作者单位:姜兆林(中国刑警学院法医系,沈阳
110035) 谭家镒(中国刑警学院法医系,沈阳 110035) 姚丽娟(中国刑警学院法医系,沈阳 110035) 邢丽梅(中国刑警学院法医系,沈阳 110035) 蒋泽良(中国刑警学院法医系,沈阳 110035) 何国标(中国刑警学院法医系,沈阳 110035) 参考文献: [1]Wong R
J.J.Anal.Toxicol.,1984, 8(1):10~14 收稿日期:2000年7月17日 修稿日期:2000年12月30日 出版日期:2001年6月1日 |
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