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摘 要：提出电堆积与等速电泳结合的毛细管电泳进样富集方法，并对电堆积和等速电泳进样富集的最佳条件进行了研究。在15kV电压，电堆积进样70s和等速电泳富集40s条件下，对两种结构相近的药物普萘洛尔和美托洛尔用毛细管区带电泳法进行了分离。pH4.0，30mmol/L醋酸钠-醋酸、30mmol/Lβ-丙氨酸-醋酸和1.5mmol/L醋酸钠-醋酸溶液分别作背景（或前导）、尾随和样品缓冲液。与常规电迁移进样方法比较，信号增强因子约为250和160；总分析时间与常规法相近。
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Abstract：A
sampling preconcentration method combining electrostacking with
isotachophoresis is presented in this paper. The optimum conditions for
electrostacking and isotachophoresis were investigated. With the
preconcentration conditions of 70 s electrostacking and 40 s
isotachophoresis, two medicines, propranolol and metoprolol, with
similar structures were separated by capillary zone electrophoresis. The
working voltage for all the steps was 15 kV. Being adjusted to pH 4.0,
30 mmol/L NaAc-HAc, 30 mmol/L β-alanine-HAc
and 1.5 mmol/L NaAc-HAc were employed as the background, viz. leading,
terminating and sample buffer solutions respectively. In comparing with
the conventional electro-migration injection, the enhancement factors in
peak height of the present method were about 250 and 160 for propranolol
and metoprolol respectively. Total analysis time was similar to that of
the conventional one. |
| 基金项目：国家自然科学基金资助项目(29975026) 作者简介：李敏(1957-)，女，理学学士，副教授，现工作单位：滁州师范专科学校化学系。 作者单位：李敏（中国科学技术大学化学系，安徽合肥 230026） 何友昭（中国科学技术大学化学系，安徽合肥 230026） 淦五二（中国科学技术大学化学系，安徽合肥 230026） 林祥钦（中国科学技术大学化学系，安徽合肥 230026） 杨丽（中国科学技术大学化学系，安徽合肥 230026） 瞿其曙（中国科学技术大学化学系，安徽合肥 230026） 参考文献： ［1］Chien R L,Burgi D S. Anal Chem,
1992,64:489-496 |
原载于《色谱》2001 Vol.19 No.2
P.176-178
http://periodical.wanfangdata.com.cn/periodical/sp/sp2001/0101/0101ml.htm