高效液相色谱法测定麻黄及其制剂中的麻黄类生物碱和川芎嗪

Abstract：A sensitive and reliable high performance liquid chromatographic method(HPLC) has been developed for the first time for the simultaneous determination of the active ingredients of ephedrine alkaloids and 2,3,5,6-tetramethyl pyrazine (TMP) in Ephedra herba crude drug and two Chinese traditional medicines (Xiao-er qingfeiwan and Lu-si kewan)． The HPLC assay was performed on a reversed phase C₁₈ column (Nova-Pak^® C18, 3．9 mm i.d.×150 mm) by using methanol-0．02 mol/L KH₂PO₄-acetic acid-triethyl amine (4∶96∶0．2∶0．01, V/V) as mobile phase for the ephedrine alkaloids analysis and methanol-H₂O-acetic acid (35∶65∶0．5,V/V) as mobile phase for TMP analysis． Regression equations revealed the linear relationships (correlation coefficients: 0．991-0．998) between the peak area of each constituent (E, PE, NE, NPE, TMP) and its concentration． The detection limits for E, PE, NE, NPE and TMP were 0．4 mg/L, 0．1 mg/L, 0．03 mg/L, 0．02 mg/L and 0．03 mg/L, respectively, and the recoveries ranged between 92%-103%． The contents of E, PE, NE, NPE, TMP in Ephedra herba, traditional medicine Xiao-er qingfeiwan and Lu-si kewan were determined respectively． The relative standard deviations (RSD) of the contents ranged between 1．1%-3%．
Keywords：high performance liquid chromatography; ephedrine(E); norephedrine(NE); pseudoephedrine(PE); norpseudoephedrine(NPE); 2,3,5,6-tetramethyl pyrazine (TMP); Ephedra