点击显示 收起
摘 要：将高效液相色谱模式/二极管阵列检测与自编紫外光谱库管理软件结合，用液相色谱/紫外光谱以及特征参数研究同种当归多个特征组分的识别及快速定量对比。色谱/光谱及其特征参数共同表达同种当归23个特征组分；探讨定量对比的关键技术；定量比较两个同种当归相同组分的浓度差异。多指标表达当归特征组分，可用于它们的识别；定量手段的建立，可对比当归特征组分定量结果的差异性。不用化学对照品，便能识别和定量比较当归样品中的多组分。方法简便易行、快速，结果的代表性强、重现性良好。
|
Abstract：A
method of multi-component quantitative analysis in root of Chinese
Angelica has been developed． The
analytical mode was reversed-phase HPLC with photodiode array detector．
Based on comparison of UV
spectra, a software of UV spectra database to assist recognition has
been established． The
solvent system, a gradient program of mobile phase with 2-propanol/acetonitrile/water
can separate a lot of liposoluble components in root of Chinese Angelica．
UV detection wavelength was
set at 202 nm and the flow rate was 1 mL/min on a Hypersil ODS column．
Twenty-three components in
two roots of Chinese Angelica were recognized using characteristic
parameters of chromatography and UV spectroscopy．
It is the base of
quantitative contrast to recognize many characteristic components
without standard components in root of Chinese Angelica．
For two samples of root of
Chinese Angelica both have 23 characteristic components and the
concentrations of some components were similar and those of others were
different． The
method is convenient and reproducible． It
can be used for the quality control of the root of Chinese Angelica
without the use of standards． |
| 作者简介：张子忠(1958-)，男，博士，山东中医药大学兼职副教授。 作者单位：张子忠（中国科学院大连化学物理研究所，辽宁大连 116011） 梁鑫淼（中国科学院大连化学物理研究所，辽宁大连 116011） 卢佩章（中国科学院大连化学物理研究所，辽宁大连 116011） 张青（中国科学院大连化学物理研究所，辽宁大连 116011） 薛兴亚（中国科学院大连化学物理研究所，辽宁大连 116011） 参考文献： ［1］L Rui-mian, HE Li-yi,
FANG Hong-ju. Acta Pharmaceutica Sinica,1980,15：371-374吕瑞绵,何丽一,方洪钜.
药学学报,1980,15：371-374 |
原载于《色谱》2001 Vol.19 No.2
P.157-160
http://periodical.wanfangdata.com.cn/periodical/sp/sp2001/0101/0101ml.htm