高效液相色谱法同时测定多种食品添加剂色谱论文 | 39康复网

摘　要：采用反相高效液相色谱法，一次进样、同时测定食品中的人工合成甜味剂（糖精钠、安赛蜜、甜味素）、防腐剂（苯甲酸、山梨酸）、咖啡因、可可碱和茶碱。以Alltech Econosphere C₁₈柱（150mm×4.6mmi.d.，3μm）为分离柱，10mmol/LNaH₂PO₄(pH4.00)-乙腈（体积比为90∶10）为流动相，采用二极管阵列检测器进行检测。整个分离过程在23min内完成。样品平均加标回收率为78.5%～107.2%。方法也可用于药品分析。

Abstract：A novel method is proposed for the simultaneous separation and determination of eight food additives, acesulfame potassium (AK), aspartame (ASP), benzoic acid (BA), caffeine (CA), saccharin sodium (SA), sorbic acid (SOR), theobromine (TB) and theophylline (TP) by reversed-phase high performance liquid chromatography． The separation was achieved within 23 min by using an Alltech Econosphere C₁₈ column with 10 mmol/L NaH₂PO₄ (pH 4．00)-acetonitrile (90∶10,V/V) as mobile phase. The qualification and quantitation were accomplished by using a photodiode array detector． The detection limits (S/N=3) for all analytes were below mg/L level． Under the experimental conditions, other common food additives and organic acids such as cyclamate, citric acid, malic acid, tartaric acid and ascorbic acid, did not interfere with the determination． The method has been successfully applied to the analysis of various foods as well as pharmaceutical preparation, and the average recoveries for real samples ranged from 78．5% to 107．2%．
Keywords：high performance liquid chromatography; food additive; synthetic sweetener; preservative; caffeine; theobromine; theophylline

基金项目：国家出入境检验检疫局科研基金资助项目（K031-1999）
作者简介：陈青川(1969-)，男，博士，工程师，电话：(010)85771629, E-mail:qchchen@hotmail.com。
作者单位：陈青川（中国进出口商品检验技术研究所，北京 100025）　
　　　　　于文莲（中国进出口商品检验技术研究所，北京 100025）　
　　　　　王静（中国进出口商品检验技术研究所，北京 100025）　

参考文献：

［1］Chen Q Ch, Mou S F, Hou X P, et al. Anal Chim Acta, 1998, 371(2-3):287-296
［2］Webb N G, Beckman D D. J Assoc Off Anal Chem, 1984, 67(3):510-513
［3］Tyler T A. J Assoc Off Anal Chem, 1984, 67(4):745-747
［4］Tsang W S, Clarke M A, Parrish F W. J Agric Food Chem, 1985, 33(4):734-738
［5］Veerabhadrarao M, Narayan M S, Kapur O, et al. J Assoc Off Anal Chem, 1987, 70(3):578-582
［6］Prodolliet J, Bruelhart M. J AOAC Int, 1993, 76(2):268-274
［7］Prodolliet J, Bruelhart M. J AOAC Int, 1993, 76(2):275-282
［8］Ostermeyer U. Dtsch Lebensm -Rundsch, 1995, 91(10):307-311
［9］Kobayashi C, Nakazato M, Ushiyama H, et al. Shokuhin Eiseigaku Zasshi, 1999, 40(2):166-171小林千种, 中里光男, 牛山博文, 等. 食品卫生学杂志(日), 1999, 40(2):166-171
［10］Chen Q Ch, Mou Sh F, Liu K N, et al. J Chromatogr A, 1997, 771(1-2):135-143
［11］Boyce M C. J Chromatogr A, 1999, 847(1-2):369-375
［12］SUN Bao-guo, HOU Peng-liang, LIN Yan-fei, et al. Journal of Analytical Science, 1999, 15(2):124-127孙保国,侯鹏亮,林雁飞,等. 分析科学学报, 1999, 15(2):124-127
［13］GB 10789-1996
［14］Windholz M. The merck index. 9th ed. Rahway: Merck, 1976.142-1 126
［15］Martell A E, Smith R M. Critical stability constants, Vol 5. New York: Plenum Press, 1982. 111
［16］Khan R. Low-calorie foods and food ingredients. London: Blackie Academic & Professional, 1993. 146

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原载于《色谱》2001 Vol.19 No.2 P.105-108
http://periodical.wanfangdata.com.cn/periodical/sp/sp2001/0101/0101ml.htm

作者：陈青川于文莲王静 2007-5-18