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| 【英文篇名】 | The study on the treatment of Oviducts Ranae protein with enzyme hydrolysis |
| 【作者】 | 李津明; |
| 【英文作者】 | LI Jin-ming（Harbin Business University; Harbin; China）; |
| 【单位】 | 哈尔滨商业大学药学院; 黑龙江哈尔滨; |
| 【刊名】 | 中国中药杂志 , 编辑部邮箱 2005年 06期 中文核心期刊要目总览 ASPT来源刊 CJFD收录刊 |
| 【英文刊名】 | China Journal of Chinese Materia Medica |
| 【中文关键词】 | 林蛙油; 酶法水解; |
| 【英文关键词】 | Oviducts Ranae; enzyme hydrolysis; pepsin; papain; |
| 【中文摘要】 | 目的:林蛙油的蛋白质由体内消化转变为体外消化,提高蛋白质利用率。方法:采取双酶水解技术对林蛙油进行水解。结果:最佳工艺条件为:底物含量0.025g·g-1,采用木瓜蛋白酶在pH7,温度60℃,酶含量0.4%,酶解3～4h后,再用胃蛋白酶水解,pH2～2.5,温度60℃,酶含量0.3%,酶解时间6～8h。结论:水解液冻干粉改善了林蛙油的溶解性和流动性,为制作各种药物制剂提供了方便。 |
| 【英文摘要】 | The paper introduced a process to enhance the yield of Oviducts Ranae protein using in vitro enzyme hydrolysis. The treatment process included two steps: (1) a 3～4 h of hydrolysis of a 0.025 g·g~(-1) concentration of substance, at pH 7 and 60 ℃, using 4% of papain; and (2) followed with a 6～8 h hydrolysis, at pH 2～2.5 and 60 ℃, using 3% of pepsin. This treatment process significantly improved the lyophilized Oviducts Ranae in solubility and fluidity, which is convenient for the relative pharmaceutical prepa... |
| 【DOI】 | CNKI:ISSN:1001-5302.0.2005-06-006 |