高密度脂蛋白抗动脉粥样硬化机制—SR-BI, CD36, PPARgamma的作用
南华大学心血管疾病研究所
易光辉
泡沫细胞形成是动脉粥样硬化发病的核心
SR-BI
动脉粥样硬化小型猪SR-BI、CD36和PPARγ表达的变化
第一部分
Figure 1: The mean body weights of the two groups throughout the study
动脉粥样硬化小型猪体重的变化
Figure 2: Plasma level of total cholesterol(a), HDL-cholesterol(b)
and triglyceride(c) in mini swine
动脉粥样硬化小型猪血脂水平的改变
A
B
Figure 3: High fat/high cholesterol diet-induced atherosclerosis in mini swine.
A: photograph of pinned-out coronary artery in control fed pigs;
B: photograph of pinned-out coronary artery in HFHC-fed pigs.
动脉粥样硬化小型猪冠状动脉苏丹Ⅳ染色
As小型猪主动脉HE染色(×20)
对照组 实验组
A
B
Figure High fat/high cholesterol diet-induced atherosclerotic plaque was stained with
hematoxylin eosin in mini swine. Magnification is ×40
A: sections (5 μm thick) were taken at the abdominal aorta in control fed pigs;
B: sections (5 μm thick) were taken at the abdominal aorta in HFHC-fed pigs.
A
B
Figure 5: High fat/high cholesterol diet-induced fatty liver was stained with
hematoxylin eosin in mini swine. Magnification is ×40
A: sections (5 μm thick) were taken at the liver in control fed pigs;
B: sections (5 μm thick) were taken at the live in HFHC-fed pigs.
动脉粥样硬化小型猪动脉及肝脏HE染色
*
GAPDH
SR-BI
M 1 2 3 4
Figure 6: Expression of SR-BI mRNA in mini swine fed with HFHC
1: Liver in control group mini swine; 2: Liver in mini swine fed with HFHC;
3: Aorta in control group mini swine; 4: Aorta in mini swine fed with HFHC;
M:Markers;*: P<0.05, compared with control group.(n=5)
SR-BI mRNA在动脉粥样硬化小型猪组织表达的变化
1 2 3 4
Figure 7: Expression of SR-BI protein in mini swine fed with HFHC.(n=5)
1: Liver in control group mini swine; 2: Liver in mini swine fed with HFHC;
3: Aorta in control group mini swine; 4: Aorta in mini swine fed with HFHC.
*: P<0.05, compared with control group.
动脉粥样硬化小型猪 SR-BI 蛋白表达的变化
A
B
Figure 8: Expression of SR-BI protein in mini swine(×40) (n=5)
A: Liver in control group mini swine; B: Liver in mini swine fed with HFHC;
M 1 2 3 4 5 6
GAPDH
596bp
CD36
246bp
1: 对照组肝脏; 3: 对照组肾脏; 5: 对照组主动脉;
2: 实验组肝脏; 4: 实验组肾脏; 6: 实验组主动脉.
* P<0.05,与对照组比较
500bp
As小型猪组织CD36 mRNA的表达变化
1 2 3 4 5 6
1: 对照组肝脏; 3: 对照组肾脏; 5: 对照组主动脉;
2: 实验组肝脏; 4: 实验组肾脏; 6: 实验组主动脉.
* P<0.05,与对照组比较
As小型猪组织CD36蛋白质的表达变化
免疫组织化学方法检测小型猪组织CD36蛋白质的表达
A: 对照组肝脏; C: 对照组肾脏; E: 对照组主动脉;
B: 实验组肝脏; D: 实验组肾脏; F: 实验组主动脉.
C
D
B
A
E
F
GAPDH
697bp
PPARγ
308bp
500bp
* P<0.05,与对照组比较
Marker Control HFHC
As小型猪主动脉组织PPARγ
mRNA的表达变化
* P<0.05,与对照组比较
Control HFHC
As小型猪主动脉组织PPARγ
蛋白质的表达变化
GAPDH
PPARγ
M 1 2 3 4
Figure 9: Expression of PPARγ mRNA in mini swine fed with HFHC
1: Liver in control group mini swine; 2: Liver in mini swine fed with HFHC;
3: Aorta in control group mini swine; 4: Aorta in mini swine fed with HFHC;
M:Markers;*: P<0.05, compared with control group.(n=5)
动脉粥样硬化小型猪肝脏、肾脏、主动脉组织SR-BI、CD36表达上调,主动脉组织PPARγ表达上调。
HDL2和HDL3对THP-1巨噬细胞SR-BI 、 CD36和PPARγ表达的影响
第二部分
HDL2和HDL3对THP-1巨噬细胞中 SR-BI表达的影响
HDL2和HDL3处理经OxLDL诱导后细胞中SR-BImRNA的表达变化
细胞中SR-BImRNA表达变化(n=3) *:Р<0.05, 与A比较;☉:Р<0.05, 与B比较; # :Р<0.05, 与C比较。 A 对照组; B OxLDL组; C 加OxLDL后换加HDL2组; D HDL2组; E 加OxLDL后换加HDL3组; F HDL3组。
后HDL2和HDL3处理经OxLDL诱导细胞中SR-BI蛋白的表达变化
细胞中SR-BI蛋白质表达变化(n=3) *:Р<0.05, 与A比较;☉:Р<0.05,与B比较; # :Р<0.05,与C比较。
A 对照组; B OxLDL组; C 加OxLDL后换加HDL2组; D HDL2组; E 加OxLDL后换加HDL3组; F HDL3组。
HDL2和HDL3对THP-1巨噬细胞 中CD36表达的影响
HDL2、HDL3与OxLDL共育细胞内CD36mRNA表达变化
细胞中CD36mRNA表达的变化(n=3) *:Р<0.05,与A比较; ☉:P<0.05,与B比较; #:Р<0.05,与C比较 。 A 对照组; B OxLDL组; C OxLDL加HDL2组; D HDL2组; E OxLDL加HDL3组; F HDL3组。
HDL2、HDL3与OxLDL共育细胞内CD36蛋白质表达变化
细胞中CD36蛋白质表达的变化(n=3)
*:Р<0.05, 与A比较; ☉:Р<0.05,与B比较; #:Р<0.05,与C比较。
A 对照组; &nbs