Lipoprotein Lipase may have a functional role in the brain
报告人：蔡嫣 Ph.D Candidate
北京大学心血管研究所
Background
The brain: the organ with the highest lipid content.
Maintenance of the special lipid composition of the neuronal membranes: vital for the proper function of the central nervous system.
Areas of particular interest in lipidology research: synthesis of the neural lipids, their transport across the blood brain barrier (BBB) and subsequent metabolism in the brain, and their removal from the brain.
Very little information on these topics of lipid transport and metabolism in the brain.
Lipoprotein Lipase Expression and Activity in Rat Brain
Michael C. Schotz et al
Journal of Lipid Research Volume 31, 1990
Eric Paradis et al JBC 2003
N2 Media alone (A & E)
40ug/ml VLDL (B & F)
10uM CuSO4 (C & G)
40ug/ml VLDL and 10uM CuSO4 (D & E)
Eric Paradis et al
Developmental Brain Research 2004
35.74±3.15
2284.21±646.9
Cultured Hippocampus Neuron of WT and LPL-/- Mice
WT
LPL-/-
Hippocampus Neuron Stained with MAP-2
WT
LPL-/-
Hippocampus Neuron Stained with Tau-2
WT
LPL-/-
Hippocampus Neuron Stained with silver nitrate
WT
LPL-/-
Future Works
Culture neurons separately from fetal and neonatal mice and differentiate primary (LPL) and secondary (hypertriglyceridemia) effect on neuronal development.