Departments of Hygiene and Epidemiology,1 Microbiology, Medical School, University of Athens,6 Hippokration General Hospital,2 Sismanoglio General Hospital,3 G. Genimatas General Hospital,4 Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece5
Received 3 March 2003/ Returned for modification 18 April 2003/ Accepted 15 May 2003
Seventeen
Klebsiella pneumoniae clinical isolates carrying the
blaVIM-1 metallo-ß-lactamase gene were collected in
the intensive care units of three hospitals in Athens, Greece,
in 2002. They exhibited various carbapenem resistance levels
(Etest MICs of imipenem ranged from 4 to 32 µg/ml). All
isolates gave positive results by the imipenem-EDTA synergy
Etest. The isolates were classified into four main types by
pulsed-field gel electrophoresis; the majority of the isolates
(5 and 10 isolates) belonged to two types. The
blaVIM-1 gene
cassette was part of the variable region of a class 1 integron
that also included
aac6,
dhfrI, and
aadA. This structure was
carried by transferable plasmids.
The metallo-ß-lactamases (MBLs) of the IMP and VIM
types are increasingly important clinically. These enzymes are
active against most ß-lactams, including carbapenems,
and have been found in various gram-negative clinical isolates
mostly in the Far East and the Mediterranean region (
12). The
wide spread of integron-borne
blaVIM genes in
Pseudomonas aeruginosa strains in Greek hospitals has been documented (
2,
9,
16). Additionally,
a sporadic strain of
Escherichia coli exhibiting resistance
to imipenem due to production of a plasmid-mediated VIM-1 MBL
was recently isolated in a Greek hospital (
10). In the present
study, we describe VIM-1-producing
Klebsiella pneumoniae clinical
strains isolated in three hospitals in Athens, Greece.
Seventeen K. pneumoniae clinical isolates were studied. They were derived from patients hospitalized from September to December 2002 in the intensive care units of three teaching hospitals (I, II, and III) located in Athens, Greece (Table 1). At least 12 of the isolates were considered causes of infection. Isolates were retained in the respective hospital laboratories because they exhibited resistance or decreased susceptibility to imipenem (a MIC of 8 µg/ml or an inhibition zone of <16 mm in diameter by the disk diffusion test) using the automated systems VITEK 2 (bioMerieux S.A.) (hospital I) and Wider I (Francisco Soria Melguizo S.A.) (hospital II) and the disk diffusion test (11) (hospital III).
fig.ommitted |
TABLE 1. Characteristics of 17 blaVIM-1-containing K. pneumoniae clinical isolates and 6 E. coli transconjugants
| |
Species identification was confirmed by using API 20E strips
(bioMerieux). MICs of ß-lactams were determined by
the Etest (AB Biodisk). Susceptibility to other antimicrobial
agents was assessed by the disk diffusion test (
11). The Etest
strip containing imipenem plus EDTA was used to detect MBL production.
Typing was performed by pulsed-field gel electrophoresis (PFGE) of XbaI-restricted genomic DNA as described previously (1). Restriction fragments were separated through a 1% agarose gel using a contour-clamped homogeneous electric field DRIII apparatus (Bio-Rad). Classification of the isolates into PFGE types was based on the criteria proposed by Tenover et al. (15).
Conjugal transfer of antibiotic resistance was performed in mixed broth cultures as described previously (17) using the E. coli strain 14R (Nalr lac) as a recipient. Transconjugant clones were selected in Mueller-Hinton agar containing nalidixic acid (100 µg/ml) plus ampicillin (50 µg/ml). Plasmid DNA preparations obtained by an alkaline lysis technique (13) were digested with PstI and electrophoresed in 0.8% agarose gels.
VIM-type genes were detected by PCR using primers VIM-F (5'-AGTGGTGAGTATCCGACAG-3') and VIM-R (5'-ATGAAAGTGCGTGGAGAC-3') corresponding to nucleotides 1339 to 1357 and 1599 to 1582, respectively, of the blaVIM-1 integron (6) (GenBank accession no. Y18050). These primers were also used to amplify a 261-bp blaVIM-1 fragment from the E. coli plasmid p541 (10). The 261-bp blaVIM-1 fragment was labeled with digoxigenin with commercially available reagents (Roche Diagnostics) and used as a blaVIM-1 probe in hybridization experiments. Mapping of class 1 integrons was performed by PCR using primers 5'CS and 3'CS (7) and a set of primers specific for various resistance genes, including aacA, dhfrI, and aadA. Nucleotide sequences of the PCR products were determined on both strands with an ABI Prism 377 DNA sequencer (Applied Biosystems).
Determination of imipenem MICs with the Etest confirmed the results reported from the hospital laboratories for 13 of the 17 isolates. The remaining four isolates (Kp5, Kp11, Kp15, and Kp16), however, exhibited an imipenem Etest MIC equal to 4 µg/ml, which is within the susceptibility range. The possible causes of this discrepancy were not investigated. All the isolates examined were multidrug resistant. MICs of imipenem ranged from 4 to 32 µg/ml. In four isolates, the MICs of imipenem were two or more doubling dilutions higher than those of meropenem. In one isolate, the MIC of meropenem exceeded that of imipenem. The MICs of other ß-lactams also varied. Seven isolates were highly resistant to aztreonam, while the MICs of aztreonam were significantly lower for the remaining isolates. The isolates were also resistant to various non-ß-lactam antibiotics, including aminoglycosides, co-trimoxazole, and chloramphenicol (Table 1). Ciprofloxacin was active against all 17 isolates.
Results of the imipenem-EDTA synergy tests indicated that all 17 isolates produced MBLs. In 10 isolates, a phantom zone of inhibition between the two gradient sections of the imipenem-EDTA Etest strip was considered a positive result (18).
The isolates were classified into four main types (A to D) by analyzing the results of PFGE. Five of six isolates from hospital I exhibited highly similar PFGE patterns (type A). Chromosomal type C accounted for the majority of the isolates from hospitals II and III. Types B and D were represented by one isolate each (Table 1 and Fig. 1).
fig.ommitted |
FIG. 1. PFGE patterns of K. pneumoniae isolates carrying VIM-type MBL genes. Lanes 1 and 2, isolates Kp1 and Kp2 (pattern A); lane 3, isolate Kp5 (pattern B); lane 4, isolate Kp6 (pattern A); lanes 5 and 6, isolates Kp7 and Kp8 (pattern C); lane 7, isolate Kp11 (pattern C1); lane 8, isolate Kp12 (pattern D); lanes 9 and 10, isolates Kp13 and Kp16 (pattern C).
| |
Conjugal transfer of resistance was attempted with seven isolates
representing the three hospitals and three PFGE types (A, B,
and C).
blaVIM-positive transconjugants were obtained from all
but one isolate (Kp5, PFGE type B). Carbapenem resistance levels
were significantly lower in the
E.
coli clones than in the donors
(Table
1). Cotransfer of resistance to non-ß-lactams
was also observed, indicating involvement of multiresistant
plasmids. Results of plasmid DNA analysis were in line with
the typing results. The plasmids derived from isolates Kp1 and
Kp2 (PFGE type A) were similar (plasmid type 1). Similar plasmids
were also obtained from the PFGE type C isolates Kp8, Kp11,
Kp13, and Kp16 (plasmid type 2). These two plasmid species were
similar in size (approximately 50 kb) but exhibited different
restriction profiles (Fig.
2A). In the digests of both plasmid
types, a band (approximately 7.5 kbp for type 1 and 5 kbp for
type 2) hybridized with the
blaVIM-1 probe (Fig.
2B).
fig.ommitted |
FIG. 2. (A) PstI restriction profiles of the blaVIM-1-carrying plasmids extracted from E. coli transconjugants trc-1, trc-2 (plasmid type 1), trc-8, and trc-13 (plasmid type 2) are presented in lanes 1 to 4, respectively. (B) Hybridization of the preparations shown in panel A with a blaVIM-1 probe.
| |
Integron mapping of seven
K.
pneumoniae isolates and the respective
E.
coli transconjugants by PCR (Table
1) indicated a common
class 1 integron structure. The cassette region was approximately
3 kb in size and contained (from 5' to 3')
blaVIM-1,
aac6,
dhfrI,
and
aadA. DNA sequencing of various overlapping PCR amplicons
from isolates Kp1, Kp8, and Kp16 and the respective
E.
coli transconjugants showed that the
aatI1 site of this class 1 integron
was followed by
blaVIM-1. The
blaVIM-1 cassette (including the
59-base element) was identical to the one in
P.
aeruginosa described
previously (
6) (GenBank accession no.
Y18050) and
E.
coli (
10).
The potential of IMP and VIM MBL genes to spread among clinical enterobacteria has also been shown previously (3-5, 14, 19-21). The results of this study, along with the recent isolation of a VIM-1-producing E. coli (10), indicate that VIM-type genes have been spread via transferable plasmids in the enterobacteria of the hospital flora in Greece. Also, acquisition of the VIM-1 integrons by distinct plasmids suggests a possible association with mobile elements. Therefore, containment of this resistance is expected to be difficult.
Decreased susceptibility to imipenem allowed recognition of the VIM-producing K. pneumoniae isolates in the hospital laboratories. Consequently, infection control measures, including isolation of these patients, were applied in a timely fashion. These measures probably accounted for the apparent decrease in the isolation frequency of imipenem-resistant K. pneumoniae strains, as indicated in the records of the three hospitals since January 2003. Typing results suggested spread of a limited number of strains. There were, however, substantial differences in the ß-lactam resistance levels between isolates of the same chromosomal type. For instance, isolate Kp16 was resistant to aztreonam, while the rest of the type C isolates were susceptible to this antibiotic. It can be hypothesized that the similarity of the PFGE patterns reflected, in some cases, strains of common origin that evolved over time.
The isolates examined may represent a portion of the blaVIM-1-containing strains circulating in these hospitals. MBL production in enterobacteria may not be able to substantially increase the MICs of carbapenems without the simultaneous operation of other mechanisms such as impaired permeability (5, 8). The differences in the imipenem MICs of the clinical isolates and transconjugants are compatible with this notion. Thus, a number of MBL-producing strains may pass unnoticed in routine susceptibility testing. Also, the imipenem-EDTA Etest strip may exhibit low sensitivity for MBL-producing enterobacteria with imipenem MICs lower than those observed here. Additionally, detection of MBL-producing enterobacteria may pose technical difficulties. As has been shown for MBL-producing nonfermenters, the composition of the medium significantly affects the MICs of carbapenems; this effect may be due to differences in zinc concentration (18). This could partly explain the discrepancies between the Etest and the methods employed in the hospitals. Therefore, standardization and evaluation of the performance of the susceptibility tests for MBL-producing enterobacteria are required.
This work was supported by the Hellenic Centre for Infectious
Disease Control (KEEL, Ministry of Health).
We thank Argiro Meni for excellent technical assistance and Kostas Gogoglou for his help.
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作者:
P. Giakkoupi A. Xanthaki M. Kanelopoulou A. Vla 2007-5-10