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Home医源资料库在线期刊临床研究杂志2005年第115卷第8期

Rap1b is required for normal platelet function and hemostasis in mice

来源:临床研究杂志
摘要:Figure1Eisincorrectinthatthelabels“Rap1a“and“Rap1b“werereversed,indicatingalackofRap1aexpressionintheRap1b-nulllane。(E)Westernblotanalysisofproteinexpressioninplateletsofindicatedgenotype。5wild-type(+/+)andRap1b-null(–/–)embryos。...

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Original citation: J. Clin. Invest. 115:680–687 (2005). doi:10.1172/JCI200522973

Citation for this corrigendum: J. Clin. Invest. 115:2296 (2005). doi:10.1172/JCI200522973C1

Credit to the co-first authors was omitted from the original manuscript. The following footnote should have appeared after the corresponding author's address.

Figure 1E is incorrect in that the labels "Rap1a" and "Rap1b" were reversed, indicating a lack of Rap1a expression in the Rap1b-null lane. The correct version of Figure 1E follows.

   Figure 1

Targeted inactivation of the rap1b gene. (A) The murine rap1b gene consists of 6 coding (bands) and 1 untranslated exon (3' UTR, open box). (B) The targeting vector contains 7.8 kb of genomic DNA flanking the neomycin-resistance cassette (neo). TK, thymidine kinase. (C) After homologous recombination, the neo cassette replaces the complete coding sequence of the rap1b gene. (D) Southern blot analysis of mouse tail DNA from heterozygous intercrosses digested with SspI and KpnI using a probe (P) that detects 5.5-kb and 9-kb fragments in the wild-type and knockout allele, respectively. (E) Western blot analysis of protein expression in platelets of indicated genotype. (F) Morphology of E15.5 wild-type (+/+) and Rap1b-null (–/–) embryos. Scale bar: 1 mm. E, EcoRI; H, HinDIII; K, KpnI; S, SspI.

 

作者: Magdalena Chrzanowska-Wodnicka, Susan S. Smyth, Si 2007-5-11
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