Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1

Original citation: J. Clin. Invest. 115:1030–1038 (2005). doi:10.1172/JCI20052396

Citation for this erratum: J. Clin. Invest. 115:2297 (2005). doi:10.1172/JCI20052396E1

During the preparation of the manuscript, the file conversion process led to the introduction of errors in the Methods section. The correct temperatures and procedures are listed below

Measurement of desaturation index. Fatty acid methyl esters were identified with an Agilent 6890 gas chromatograph connected to an HP-5 column (30 m x 0.32 mm x 0.25 μM film thickness) connected to a flame ionization detector set at 250°C. The column and the injector temperature were set to 50°C. The column temperature was increased to 150°C at 4°C/min, increased to 200°C at 3°C/min, held at 200°C for 25 minutes, and then increased to 225°C at 25°C/min and held at 225°C for 10 minutes. Under these conditions, the 9-16:1, 16:0, 9-18:1, and 18:0 methyl esters eluted at 14.2, 14.8, 19.4, and 20.2 minutes, respectively. The desaturation index was calculated as the ratios of 9-16:1/16:0 and 9-18:1/18:0.