CT如何对软骨细胞起作用的精确的信号传导途径及软骨细胞本身在增殖分化过程中的信号传导途径在降钙素作用下的变化应是以后研究的重点。
通过本实验可以看出降钙素5 IU／（kg·g)皮下注射能够增加体外培养软骨细胞分泌的Aggrecan的含量以及下调MMP-3的表达；可能通过体内明显减轻兔膝关节软骨基质的降解，减少MMP-3的表达而保护软骨。
【参考文献】
〔1〕 Burrage PS,Mix KS,Brinckerhoff CE.Matrix metalloproteinases:role in arthritis［J］.Front Biosci,2006,11:529-543.
〔2〕 李任增，张柳.降钙素对兔骨关节炎关节软骨的保护作用［J］.中国矫形外科杂志，2006，14：121-123.
〔3〕 刘志翔，张柳，张楠.降钙素对兔骨关节炎软骨基质金属蛋白酶1的影响［J］.中国矫形外科杂志，2006，14：1741-1743.
〔4〕 Benbow U,Brinckerhoff CE.The AP-1 site and mmp generegulation:what is all the fuss about［J］.Matrix Biol,1997,15:519-526.
〔5〕 Elders MJ.The increasing impact of arthritis on public health［J］.J Rheumatol Suppl,2000,60:6-8.
〔6〕 Malfait AM,Liu RQ,Ijiri K,et al.Inhibition of ADAM-TS4 and ADAM-TS5 prevents aggrecan degradation in osteoarthritic cartilage［J］.J Biol Chem,2002,277:22201-22208.
〔7〕 Nelson KK,Melendez JA.Mitochondrial redox control of matrix metalloprotei-nases［J］.Free Radic Biol Med,2004,37:768-784.
〔8〕 Vankemmelbeke MN,Jones GC,Fowles C,et al.Selective inhibition of ADA-MTS-1,-4 and-5 by catechin gallate esters［J］.Eur J Biochem,2003,270:2394-2403.
〔9〕 Cranney A,Tugwell P,Zytamk N,et al.Meta-analyses of therapies for postmen-opansal osteoporosis.VI.Meta-analysis of calcitonin for the treatment of postmenopausal osteoporosis［J］.Endoer Rev,2002,23:540-551.
〔10〕 Hajjaji H,Williams JM,Devogelaer JP,et al.Treatment with calcitonin prevents the net loss of collagen,hyaluronan and proteoglycan aggregates from cartilage in the early stages of canine experimental osteoarthritis［J］.Osteoarthritis Cartilage,2004,12:904-911.
〔11〕 Sondergaard BS,Wulf H,Henriksen K,et al.Calcitonin directly attenuates collagen Type Ⅱ degradation by inhibition of MMP expression and activity in articular chondrocytes［J］.Osteoarthritis Cartilage,2005,Suppl A:12.
作者单位：华北煤炭医学院附属医院骨科，河北唐山 063000