摘要:) 1M IPTG M9 media (minimal media)1 Liter 5x M9 media: (sterile filtered)30g Na2HPO4 or 64g Na2HPO4-7H2O 15g KH2PO4 5g NH4Cl 2。 20% glucose (sterile filtered or autoclaved) 1M MgSO4 (sterile filte...
08-02-03摘要:Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginaseSource: Hikmet Geckil‘s LabAbstract: This is a fast, efficient and reproducible recovery procedure for p...
08-02-03摘要:Biosynthetic labelingHow long should cells be labeled。 The ideal length of time to label cells depends on the protein of interest and the label that you are using。 If you want to label an unstabl...
08-02-03摘要:Amicon Stirred Ultrafiltration Cells (Models 8050, 8400) Description - Membranes - Assembly - Operation - Limits - Sterilization - Maintenance - Troubleshooting DescriptionFor protein concentration...
08-02-03摘要:Acetylation (or Succinylation) of Amino Groups on ProteinsREFERENCE: Hanock and Benz。25mg/ml (diluted in 50mM phosphate buffer pH 6。pH microprobe to monitor pH during acetylation reaction。Using ...
08-02-03摘要:Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O。 3) Just before use, add 50 ml acetic acid to 10% final concentration。 4) Stain for 2 hours。 Staining time can be sh...
08-02-03摘要:(1)放射性碘源的选用:无载体的131I或125I均可用于碘化标记,但应尽量选用新鲜的、比放射强度高的、含还原剂量少的放射性碘源。碘源的比放射强度最好≥50~100mCi/ml,至少也要30mCi/ml,否则加入碘源的容量要增...
08-02-03摘要:本法反应温和,对抗原、抗体免疫活性影响小,已被广泛应用。1.原理此法是利用乳过氧化物酶(Lactoperoxidase)有促进微量过氧化氢对125I-的氧化作用,生成125I+,并标记在多肽、蛋白质酪氨酸分子上。2.方法以标...
08-02-03摘要:1.原理用Iodogen为氧化剂,对蛋白质和多肽抗原进行碘化标记,把125I直接引进分子中的酪氨酸残基上。标记过程中被标记样品不与Iodogen混合,标记后取出样品即停止反应,不使用任何还原剂。2.方法(1)标记之前,...
08-02-03摘要:Immerse gel in 50% ethanol/10% acetic acid for at least 1 hr。 Soak in 5% ethanol/5% acetic acid overnight or for a minimum of 2 hours。 Wash in diH2O for 1 hr。 Wash in diH2O twice, 15 min each。
08-02-03