Biorad Protein Assay: Bradford

Biorad Protein Assay: Bradford

Standards: 1 mg/ml BSA stock

- dilute 1:10 to get 0.1 mg/ml BSA

Add To get H-2O

20 µl 2 µg/ml 780 µl

40 µl 4 µg/ml 760 µl

60 µl 6 µg/ml 740 µl

80 µl 8 µg/ml 720 µl

100 µl 10 µg/ml 700 µl

120 µl 12 µg/ml 680 µl

Protocol:

1) Add 1, 2, 3, or 4 µl of concentrated unknown, and bring volume up to 800 µl with water.

2) Add 200 µl concentrated Biorad reagent and incubate at room temperature for 5 minutes.

3) Assay absorbance at 595 nm.