Lowry Protein Assay
reagents
- 2% Na2CO3 in 0.1 N NaOH
- 2% NaK Tartrate
- 1% CuSO4•5H2O
Mixture before use: 25.00 ml of 1
0.25 ml of 2
0.25 ml of 3
- 2N Folin reagent (yellow in color)
- Standard: Given BSA 1 mg/ml
procedure
- Transfer sample containing up to 40 µg of protein to test tubes and bring volume up to 200 µL with H2O.
- Add 1 mL of mixture and mix well with the Vortex.
- Incubate for 10 minutes.at Room Temperature.
- Add 50 µL of Folin reagent, mix well with Vortex.
- Incubate for 30 minutes at Room Temperature.
- Read at 540 nm against an H2O blank.
作者:
佚名 2008-2-3