毛细管电泳――基因突变及多态性分析新方法

摘　要：摘要着重介绍基因突变及多态性分析方法以及毛细管电泳在该领域中的应用。主要包括单链构象多态性分析，变性梯度及温度梯度电泳，杂合子分析，限制性片段多态性分析，等位基因特异性扩增，核酸杂交，引物扩展及小卫星和微卫星分析。

关键词：毛细管电泳，激光荧光，基因突变，聚合酶链反应，评述

分类号：O65　文献标识码：A

Capillary Electrophoresis――An Attractive Alternative Tool for Analyses ofGenetic Mutations/Polymorphisms

Ren Jicun（College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081）　

Abstract：Recently, capillary electrophoresis (CE) has been successfully used for genetic analysis instead of conventional slab gel electrophoresis. This article will give an overview of the fundamental aspects on mutation/polymorphism analyses in combination with capillary electrophoresis (CE), which mainly includes single strand conformation polymorphism analysis, denaturing gradient-gel electrophoresis, heteroduplex analysis, chemical mismatch cleavage, restriction fragment length polymorphism, allele specific oligonucleotide hybridization, allele specific amplification, primer extension, and minisatellite and microsatellite analyses. A number of key applications are summarized.

Keywords：Capillary electrophoresis, laser-induced fluorescence, genetic mutaion, polymerase chain reaction, review

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收稿日期：2000年4月17日

修稿日期：2000年9月30日

出版日期：2001年4月1日