α1-酸性糖蛋白柱拆分沙美特罗对映体

摘　要：目的：建立沙美特罗对映体拆分方法。方法：以α₁-酸性糖蛋白(α₁-AGP)为固定相，考察了流动相pH、有机改性剂及流速对分离的影响。结果：以10mmol^.L^-1醋酸钠(pH4.0)-异丙醇(98∶2)为流动相，流速为0.9mL^.min^-1，在α₁-AGP柱上拆分了沙美特罗。结论：流动相pH和有机改性剂的量是优化分离带正电荷对映体的分离的重要因素。同时可以在一定的流速范围内通过调节流速来提高柱效、改善分离。

关键词：沙美特罗　对映体　手性拆分　α₁-酸性糖蛋白

分类号：R927　文献标识码：A

Enantiomeric Separation of Saklmererol by HPLC Using a-Acid Glycoprotein Chiral Atationary Phase

YANG Yong-jina（Shanghai Inertitute for Drong Control Shanghai 200233）　
HOU Mei-qin（Shanghai Inertitute for Drong Control Shanghai 200233）　

Abstract：To develop an enantionmeric sepatration mentod of salmeterol by HPLC using -acid glycopratein (a₁-AGP )chiral stationary phase Method :Indluences of the pH value of the mobile phase organic modifiers,ans floe rate on the enantionmetric sparation of salmerterol using a₁-AGP chiral straruionary phase were investigared Resulte :Salmeeterol enanhtiometric were separated on an a₁-AGP chiral statonary phase colunmn with a moblie phase constsed of to mmol^.l^-1 NaAc(pH4.0)-isopropanol (98:2) at a flow rate of 0.9 ml^.min^-1 Conclustion :The pH value of the mobile phase and teh organic modifier concentration were vwery imporation parameteers when optimzing the enantomene sep-aration of thew cationic racem,metes Higher column efficiency was obtainde with lower flow rates

Keywords：salmertero;l enantio,mers enantiomeric separation a₁-AGP

参考文献：

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收稿日期：2000年3月6日

出版日期：2001年3月31日