摘 要:借鉴高分子亚浓溶液线团收缩理论,研究了脱氧核糖核酸(DNA)片段在高分子溶液全浓度区间的电泳迁移行为。结果表明,在高分子稀溶液、亚浓溶液和浓溶液3个不同浓度区间,DNA的电泳迁移行为各不同,DNA片段的分离在这3个浓度区间也存在差异。
Abstract:The theory of polymer coils shrinking in semi-dilute solu tion has recent
ly been developed on polymer solution. The polymer solution from coils s hrinki
ng concentration CS to uniform entangled concentration C+
has been d efined as semi-dilute solution. We experimentally investigated the
electrophoretic migration behavio r of 100 bp deoxyribonucleic acid (DNA) Ladder
in hydroxypropyl methyl cellulose (HPMC) concentration ran ging from 1.25 g/L to
16.06 g/L. The friction force mobility μf is used to expr ess the friction
force that DNA will encounter in capillary electrophoresis. Our results indicate
the division of polymer solution into three regions depending on the
relationship of μf and HPMC concentration and Ferguson plot. Resolutions of 200 bp/300 bp
and 700 bp/800 bp show semi-dilute polymer solution suits large fragments and
small fragments DNA separation r espectively. The result s confirm that the
current polymer theory is valid under actual CE
condition. Keywords:capillary electrophoresis; deoxyribonucleic acid;
the ory of coil shrinking in semi-dilute polymer
solution |
作者简介:靳 艳(1968-),女,博士研究生。
作者单位:靳艳(中国科学院大连化学物理研究所, 辽宁 大连
116023) 林炳承(中国科学院大连化学物理研究所, 辽宁 大连 116023) 冯应升(香港大学 化学系,
香港)
参考文献:
[1] Zhu M D,Hansen D L,Burd S,et al. J
Chromatogr,1989,480:311-319 [2] Sunada W M,Blanch H W.
Electrophoresis,1997,18:2 243-2 254 [3] Barron A E,Blanch H W,Soane D S.
Electrophoresis,1994,15:597-615 [4] Pariat Y F,Berka J,Heiger D N,et al. J
Chromatogr A,1993,652:57-66 [5] Fung E N,Yeung E S. Anal Chem,1995,67:1 913-1
919 [6] McGregor D A, Yeung E S. J Chromatogr A, 1993,652:67- 73 [7]
Chrambach A,Radko S P. Electrophoresis, 2000,21:259-26 5 [8] Qian R Y.
Exicimer studies of polymer solutions over the entire concentration range.
In:Lee L H,ed. New trend in physics and physical chemistry of polymer. New
York:Plenum Press,1989.239-253 [9] De Gennes P G. Scaling concepts in polymer
physics. Ithaca:Cornell University Press, 1979.63-66 [10] Grossman P D, Soane
D S. Biopolymers, 1991,31:1 221-1 228 [11] GUO Xu, XUE Jun, LIN Bing-cheng.
Bulletin of Science and Technology, 1996,41(12):1 090-1 094 郭 栩,薛 俊,林炳承.
科学通报, 1996,41(12):1 090-1 094 [12] Barron A E, Soane D S, Blanch H W. J
Chromatogr A, 1993,652:3- 16 [13] QIAN Ren-yuan,CAO Ti,CHEN Shang-xian,et al.
Chinese Sciences, Series B,1983,12:1 080-1 087 钱人元,曹 锑,陈尚贤. 中国科学*B辑,1983,12:1 080-1 087 [14] Lohse D J.
Maromolecules,1981,14:1 658-1 667 [15] Cheng R S, Yan X H. J Appl Polymer
Sci: Appl ied Polmer Symposium,1991,48:123-132 [16] Qian R Y, Cao T. Polymer
Communications,1986,127:169-171
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原载于《色谱》2001 Vol.19 No.1 P.60-63
http://periodical.wanfangdata.com.cn/periodical/sp/sp2001/0101/0101ml.htm
作者:
靳艳 林炳承 冯应升 2007-5-18