新型磁性葡聚糖亲和吸附剂的制备及在尿激酶纯化中的应用

Abstract：The reverse phase suspension and embedment technique were a dopted to pre pare magnetic dextran microsphere (MDMS). The dispersion medium was mixture of some organic solvents. Span-80 was used as stabilizer. The aqueous dextran wit h magnetic fluid was suspended in dispersion medium with epichlorohydrin as cross -linking reagent. The mixture was stirred for 30 minutes at room temperature a nd then he ated at 70 ℃ for 4 hours, MDMS was thus obtained. MDMS was activated by epich lor ohydrin on which 6-aminohexanoic acid, glycine or ethylene diamine was bonded a s spacers. Then it was coupled with p-aminobenzamide, L-arginine methy l ester or guanidohexanoic acid and five magnetic affinity adsorbents were prepared. The M D MS was polydisperse particles with the size of 50-300 meshes and the con tent of Fe₃O₄ was about 6.2 per cent in the MDMS. Influence of some parameters suc h as visc osity and density of organic phase, the volume ratio of organic and aqueous phas e, the quantity of surfactant and stirring speed on preparing MDMS was studied. Magnetic affinity adsorbents were used to purify crude urokinase in a batch mode and the effect of coupling reagents and ligands on results of purification was disc ussed. The bioactivity recovery was 40.0 to 60.7 per cent, the purification- fold was between 14.9 and 32.8, and the adsorptive capacity varies from 89 mg to 121 mg per milliliter of adsorbent.
Keywords：dextran; magnetic affinity adsorbent; preparation; u rokinase; purification