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【英文篇名】 | Fingerprint of Flos Lonicerae by HPLC-FPC |
【作者】 | 王倩; 王建新; 于治国; 胡馨; 张英华; |
【英文作者】 | WANG Qian 1; WANG Jian-xin 1*; YU Zhi-guo 2; HU Xin 1; ZHANG Ying-hua 1 (1.Shanghai Institute of Traditional Chinese Material Medical; Shanghai; 2. Department of Pharmaceutical Analysis; School of Pharmacy; Shenyang Pharmaceutical University; Shenyang; China); |
【单位】 | 上海中药研究所; 沈阳药科大学药学院药物分析教研室; 辽宁沈阳; |
【刊名】 | 中成药 , 编辑部邮箱 2005年 07期 中文核心期刊要目总览 ASPT来源刊 CJFD收录刊 |
【英文刊名】 | Chinese Traditional Patent Medicine |
【中文关键词】 | 金银花; 指纹图谱; 高效液相色谱; |
【英文关键词】 | Flos Lonicerae; fingerprint chromatographic; HPLC; gradient elution; |
【中文摘要】 | 目的:建立金银花药材HPLC指纹图谱。方法:用梯度洗脱法,对金银花药材进行HPLC测定,流动相为1醋酸溶液甲醇线性梯度洗脱,检测波长为254nm。结果:运用梯度洗脱能很好分离金银花的各类成分;经方法学考察,HPLC指纹图谱具有良好的重现性:10批样品中11个特征指纹峰有专属性。结论:本研究建立的金银花药材HPLC指纹图谱分析法可用于金银花药材质量的综合评价。 |
【英文摘要】 | AIM To establish the HPLC-FPC of Flos Lonicerae . from different growing areas. METHODS The gradient was adopted elution with methanol and 1 % acetic acid, at detection wavelength was at 254 nm. RESULTS The constitutents of Flos Lonicerae were well separated on HPLC, and the HPLC-FPC of 10 batchs of samples was established. The results showed that this method had a good repeatability, and the 11 common peak’s had an exclusive properties. CONCLUSION The method developed can evaluate the quality of Flos Lonic... |
【DOI】 | CNKI:ISSN:1001-1528.0.2005-07-002 |