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不同剂量白细胞介素2作为乙型肝炎病毒核酸疫苗佐剂的效应
免疫学杂志 2001年第1期第17卷 基础免疫学
作者:李文波 姚志强 周永兴 权启镇 冯志华
单位:李文波 权启镇(济南军区总医院消化科,山东 济南 250031);姚志强 周永兴 冯志华(第四军医大学唐都医院感染病科,陕西 西安 710038)
关键词:白细胞介素2;肝炎病毒;乙型;核酸疫苗
摘 要:目的 构建表达乙型肝炎病毒(HBV)S蛋白的重组质粒pCR3.1-S作为核酸疫苗,观察重组人白细胞介素2(rhIL-2)作为佐剂对其诱导BALB/c小鼠产生免疫应答的影响。方法 分别以ELISA法、3H-TdR掺入法、Cr51 4h释放法等测定免疫小鼠的血清抗HBs、淋巴细胞增殖活性、淋巴细胞杀伤效应,初步研究不同免疫组体液和细胞免疫应答的差异。结果 血清抗HBsELISA效价,rhIL-2组与对照组相比较差异不显著(P>0.05);淋巴细胞增殖指数、淋巴细胞杀伤率,1000U(只.次)rhIL-2/组和2000U(只.次)rhIL-2/组与对照组相比较差异显著(P<0.05),而500U(只.次)rhIL-2/组与对照组相比较无显著差异(P>0.05)。结论 一定剂量的rhIL-2作为佐剂可增强HBV核酸疫苗的免疫效应。
分类号:R512.6 文献标识码:A
文章编号:1000-8861(2001)01-0027-03
Effects of different doses of interleukin 2 as adjuvants on immune responses induced by nucleic acid vaccines of hepatitis B virus
LI Wen-bo(Department of Gastroenterology, General Hospital of Jinan Military Region, Jinan, 250031,China)
YAO Zhi-qiang(Department of Infectious Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi’an 710038, China)
ZHOU Yong-xing(Department of Infectious Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi’an 710038, China)
QUAN Qi-zhen(Department of Gastroenterology, General Hospital of Jinan Military Region, Jinan, 250031,China)
FENG Zhi-hua(Department of Infectious Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi’an 710038, China)
Abstract:Objective To construct recombinant plasmids of pCR3.1-S encoding protein S of hepatitis B virus as nucleic acid vaccines and investigate the effects of recombinant human interleukin 2 as adjuvants on immune responses induced by HBV nucleic acid vaccines in BALB/c mice. Methods Anti-HBs antibodies to HBV major protein was measured by ELISA assay, and the activities of lymphocyte proliferation and cytotoxity were detected by 3H-TdR incorporation assay and 4-hour 51Cr release assay. Humoral and cellular immune responses of different groups were then compared. Results The titers of anti-HBs antibodies in serum of rhIL-2 group mice were not significantly higher than those of control group mice (P>0.05). The difference of the activities of lymphocyte proliferation and cytotoxity between of control group mice and rhIL-2 group mice (every mouse with 500 U one time) was not significant (P>0.05). However, stimulation index and specific lysis rate of lymphocyte of rhIL-2 group mice (every mouse with 1 000 U or 2 000 U one time) showed significant difference compared with htose of control group mice (P<0.05). Conclusion Recombinant plasmids of pCR3.1-S as HBV nucleic acid vaccines could induced strong humoral and cellular immune responses in BALB/c mice. rhIL-2 acts as adjuvants to increase the potency of HBV nucleic acid vaccine in some certainty.
Keywords:interleukin 2; hepatitis B virus; nucleic acid vaccine基金项目:国家自然科学基金资助项目(39770665)
作者简介:李文波(1974-)男,山西洪洞县人,硕士,住院医师,主要从事乙型肝炎病毒核酸疫苗的研究。Tel:(0531)2187681-66066;E-mail:wenboligood@263.net参考文献:
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收稿日期:2000年3月20日
修稿日期:2000年9月27日
出版日期:2001年1月15日