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摘 要：目的探讨营养不良、肾病本身和糖皮质激素作为各自独立因素对大鼠血清胰岛素样生长因子1(IGF-1)浓度的影响，阐明血清IGF-1代谢紊乱与肾病综合征(NS)大鼠生长障碍的关系。方法24只周龄相同体重相近的雄性SD大鼠被随机分成正常对照、食物对照、阿霉素肾病和地塞米松治疗的阿霉素肾病4组。血清IGF-1浓度和肝脏ICF-1Mrna表达分别采用放射免疫(RIA)和逆转录-聚合酶链反应(RT-PCR)法检测。结果1．食物对照和肾病组大鼠较正常组血清IGP-1明显减低，肝脏IGF-1Mrna表达明显增高，但两组间差异显著性学意义。2．激素治疗组大鼠较肾病组血清IGF-1及肝脏IGF-1Mrna表达均显著下降。3．血清IGF-1浓度与大鼠鼻-尾长度和体重均呈正相关(P＜0．01)。结论肾病综合征生长障碍归因于继发性营养不良所致的血清IGF-1浓度减低，糖皮质激素治疗则通过进一步减低血清IGF-1浓度使生长障碍加剧。
Serum IGF-1 and liver IGF-1 mRNA in nephrotic rats with growth failure
YI Zhuwen ,LIU Jianhua
(Department of Pediatrics,Second Affiliated Hospital,Hunan Medical University,Changsha 410011,China)
Abstract：Objective To investigate the effects of malnutrition,nephrosis itself and glucocorticoid therapy on serum IGF-1 and liver IGF-1 mRNA,and illustrate relationship between growth failure in nephrotic rats and turbulence of serum IGF-1.Methods Twenty-four male SD rats were randomly divided into cuntrol,pair-fed,doxorubincin-induced nephrotic(nephrotic) and dexamethasone-treated nephrotic (des-treated) rats.Serum IGF-1 and liver IGF-1 mRNA were measured by RIA and RT-PCR respectively.Results 1.Sermn IGF-1 was reduced and liver IGF-1 mRNA was increased in pair-fed and nephrotic rats,but no significant difference was found between two groups.2.Serum IGF-1 and liver IGF-1 mRNA were lower in des-treated rats than in nephrotic rats.3.There was a positive correlation of serum IGF-1 with nose-tail length and weight.Conclusions Reduced serum IGF-1 induced by secondary malnutrition is the cause of growth failure in nephrotic syndrome.Glucocorticoid therapy deteriorates growth failure in nephrotic syndrome by further decreased liver IGF-1 synthesis.
Keywords：Growth disorders;Nephrotic syndrome;Insulin-like growth factor
基金项目：湖南省自然科学基金资助项目[湘科计字(97)37号-17]
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