摘要：auABSTRACTThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied inlysates and cell extracts of Helicobacter pylori by three different methods。 Nuclear ...

摘要：Indexing terms: d amino acid oxidase。 methods。 AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are ...

摘要：5 - 10 µ。Add HCl to a final concentration of 2N and a final volume of 0。6 ml。Prepare standards containing 0 - 20 µ。

摘要：Enzyme Kinetics assay of the WT To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media Inc。, Lavellet...

摘要：In cases of programmed cell death, such as the formation of aerenchyma (large air spaces in the cortex of plants in flooded soils), and in the abscission zones of leaves and fruits, cellulases are ...

摘要：The assayProtease enzymes are added to a milky colloidal suspension of egg albumen。 As the protease enzymes digest the suspended particles of proteins, the mixture becomes more transparent。 The a...

摘要：Polyphenoloxidase (catechol oxidases) assayBrowning of the cut surface of some fruits and vegetables is due the presence of a group of enzymes called polyphenoloxidases。One example of a substrate ...

摘要：Polygalacturonase assayThis enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page)。 The cells of these tomatoes have b...

摘要：Pectinase assayPectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are widely used to help extract, clar...

摘要：Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose ChromatographyJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W。 RussellUniversity of Texas Southwe...

摘要：Identification of a Mutant Kinase/ATP Analog PairScott T。 Determining the direct substrates of protein kinases is important in understanding how these signaling enzymes exert their effects on cell...

摘要：Phosphorylation of SubstratesScott T。 Eblen, N。 Vinay Kumar, and Michael J。 WeberDepartment of Microbiology and Cancer Center, University of Virginia Health System, Charlottesville, VA 22908Exce...

摘要：cAMP AssaysGouzel Karimova and Daniel LadantUnite Postulante de Biochimie des Interactions Macromoleculaires, Departement de Biologie Structurale et Chimie, CNRS URA 2185, Institut Pasteur, Paris, ...

摘要：一、ATP的生成方式体内ATP生成有两种方式(一)底物水平磷酸化(substrate level phosphorylation)底物分子中的能量直接以高能键形式转移给ADP生成ATP，这个过程称为底物水平磷酸化，这一磷酸化过程在胞浆和线粒体中...

摘要：蛋白质与多肽激素的放射免疫分析第一节概述1960年，美国学者Yalow 和Berson 创立了放射免疫分析（Radioimmunoassay，RIA），并首先用于糖尿病人血浆中胰岛素含量的测定。放射免疫分析的物质，由激素扩大到几乎一切...