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Asymmetric sequences are indicated by *.
Single letter code:
R = G or A;
Y = C or T;
W = A or T;
M = A or C;
K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;
B = C, G or T;
D = A, G or T;
N = G, A, T or C.
Recognition
sequenceEnzyme 5'-protruding end (5'=>3') 1nt 2nt 3nt 4nt 5nt AA^CGTT Psp1406I CG A^AGCTT HindIII AGCT ^AATT TasI AATT A^CATGT BspLU11I CATG A^CCGGT BshTI CCGG ACCTGC(4/8)^* BveI NNNN A^CCWGGT SexAI CCWGG A^CGCGT MluI CGCG A^CRYGT AflIII CRYG A^CTAGT BcuI CTAG A^GATCT BglII GATC AT^CGAT Bsu15I CG AT^TAAT VspI TA C^AATTG MunI AATT CACCTGC(4/8)^* AarI NNNN CACGAG(-5/-1)^* BssSI ACGA CA^TATG NdeI TA ^(13/9)CATCC* FokI NNNN C^CATGG NcoI CATG CCCGC(4/6)^* SmuI NN C^CCGGG Cfr9I CCGG CCGC(-3/-1)^* AciI CG C^CGG HpaII CG C^CGG MspI CG ^CCNGG BssKI CCNGG CC^NGG Bme1390I N C^CNNGG BseDI CNNG C^CRYGG DsaI CRYG CC^SGG BcnI S C^CTAGG XmaJI CTAG CCTCAGC(-5/-2)^* BbvCI TCA CCTNAGC(-5/-2)^* Bpu10I TNA CC^TNAGG Eco81I TNA CCTNN^NNNAGG XagI N CC^WGG MvaI W C^CWWGG Eco130I CWWG C^GGCCG Eco52I GGCC CG^GWCCG CpoI GWC C^GTACG Pfl23II GTAC CGTCTC(1/5)^* Esp3I NNNN C^YCGRG Eco88I YCGR CR^CCGGYG SgrAI CCGG C^TAG FspBI TA C^TCGAG XhoI TCGA CTCGTG(-5/-1)^* BssSI TCGT CTCTTC(1/4)^* Eam1104I NNN C^TYRAG SmiI TYRA C^TNAG DdeI TNA C^TRYAG BfmI TRYA C^TTAAG BspTI TTAA
Recognition
sequenceEnzyme 5'-protruding end (5'=>3') 1nt 2nt 3nt 4nt 5nt GAAGAC(2/6)^* BpiI NNNN ^(4/1)GAAGAG* Eam1104I NNN ^(4/1)GAAGAGC* SapI NNN G^AATTC EcoRI AATT GACGC(5/10)^* HgaI NNNNN GACN^NNGTC PsyI N ^(5/4)GACTC* PleI N ^(5/1)GAGAC* Alw26I NNNN ^(5/1)GAGACC* Eco31I NNNN ^(5/1)GAGACG* Esp3I NNNN GAGTC(4/5)^* PleI N G^ANTC HinfI ANT ^GATC Bsp143I GATC ^GATC MboI GATC ^(5/4)GATCC* BspPI N ^(9/5)GATGC* LweI NNNN G^AWTC TfiI AWT GCAGC(8/12)^* BseXI NNNN ^(8/4)GCAGGT* BveI NNNN GCATC(5/9)^* LweI NNNN G^CGC Hin6I CG G^CGCGC PauI CGCG GCGG(-3/-1)^* AciI CG GC^GGCCGC NotI GGCC ^(6/4)GCGGG* FauI NN ^(10/5)GCGTC* HgaI NNNNN GC^NGC SatI N G^CTAGC NheI CTAG GCTCTTC(1/4)^* SapI NNN GCTGAGG(-5/-2)^* BbvCI TGA ^(12/8)GCTGC* BseXI NNNN GC^TNAGC Bpu1102I TNA GCTNAGG(-5/-2)^* Bpu10I TNA G^CWGC TseI CWG GGATC(4/5)^* BspPI N G^GATCC BamHI GATC GGATG(9/13)^* FokI NNNN GG^CGCGCC AscI CGCG GGGAC(10/14)^* BsmFI NNNN G^GGCCC Bsp120I GGCC GG^GWCCC SanDI GWC G^GYRCC BshNI GYRC G^GNCC Cfr13I GNC G^GTACC Acc65I GTAC GGTCTC(1/5)^* Eco31I NNNN G^GTNACC Eco91I GTNAC G^GWCC Eco47I GWC GR^CGYC Hin1I CG G^TAC Csp6I TA ^(14/10)GTCCC* BsmFI NNNN G^TCGAC SalI TCGA GTCTC(1/5)^* Alw26I NNNN ^(6/2)GTCTTC* BpiI NNNN G^TGCAC Alw44I TGCA GT^MKAC XmiI MK ^GTNAC MaeIII GTNAC ^GTSAC NmuCI GTSAC
Y, R, T, W
Recognition
sequenceEnzyme 5'-protruding end (5'=>3') 1nt 2nt 3nt 4nt 5nt Y^GGCCR CfrI GGCC R^AATTY XapI AATT R^CCGGY Cfr10I CCGG R^GATCY PsuI GATC RG^GNCCY EcoO109I GNC RG^GWCCY Psp5II GWC T^CATGA PagI CATG T^CCGGA Kpn2I CCGG T^CCNGGA PfoI CCNGG T^CGA TaqI CG T^CTAGA XbaI CTAG T^GATCA BclI GATC T^GTACA Bsp1407I GTAC T^TAA Tru1I TA TT^CGAA Bsp119I CG W^CCGGW BsaWI CCGG W^GTACW TatI GTAC