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Asymmetric sequences are indicated by *.
Single letter code:
R = G or A;
Y = C or T;
W = A or T;
M = A or C;
K = G or T;
S = C or G;
H = A, C or T;
V = A, C or G;
B = C, G or T;
D = A, G or T;
N = G, A, T or C.
Recognition
sequenceEnzyme 3'-protruding end (5'=>3') 1nt 2nt 3nt 4nt 9nt ACGT^ TaiI ACGT ACN^GT TaaI N ACTGG(1/-1)^* BseNI GN ACTGGG(5/4)^* BfiI N ATGCA^T Mph1103I TGCA CACNNN^GTG AdeI NNN ^(0/2)CACTGC* BtsI NN CAGNNN^CTG CaiI NNN CASTG(2/-7)^ TspRI NNCASTGNN ^(0/2)CATCC* BseGI NN CATG^ NlaIII CATG ^(0/2)CATTGC* BseMI NN ^(-1/1)CCAGT* BseNI NC CCANNNNN^NNNNTGG XcmI N CCANNNNN^NTGG BstXI NNNN CCANNNN^NTGG Van91I NNN ^(4/5)CCCAGT* BfiI N CCGC^GG Cfr42I GC CCNNNNN^NNGG BseLI NNN CCTC(7/6)^* MnlI N CCTGCA^GG SdaI TGCA CGAT^CG PvuI AT CGRY^CG Bsh1285I RY CTCAG(10/8)^* BseMII NN ^(14/16)CTCCAG* GsuI NN ^(8/10)CTCCTC* BseRI NN CTGAAG(16/14)^* Eco57I NN CTGRAG(16/14)^* Eco57MI NN ^(8/10)CTGAG* BseMII NN ^(14/16)CTGCAC* BsgI NN CTGCA^G PstI TGCA CTGGAG(16/14)^* GsuI NN ^(14/16)CTTCAG* Eco57I NN
G, R, T
Recognition
sequenceEnzyme 3'-protruding end (5'=>3') 1nt 2nt 3nt 4nt 9nt GAAGA(8/7)^* MboII N GAATGC(1/-1)^* Mva1269I CN GACGT^C AatII ACGT GACNNN^NNGTC Eam1105I N GACNNNN^NNGTC AasI NN GAGCT^C SacI AGCT ^(6/7)GAGG* MnlI N GAGGAG(10/8)^* BseRI NN GCAATG(2/0)^* BseMI NN GCAGTG(2/0)^* BtsI NN GCANNNN^NTGC BstAPI NNN GCATG^C PaeI CATG ^(-1/1)GCATTC* Mva1269I NG GCCNNNN^NGGC BglI NNN GCGAT^CGC SgfI AT GCNNNNN^NNGC HpyF10VI NNN GDGCH^C SduI DGCH ^(5/6)GGATAC* BfuI N GGATG(2/0)^* BseGI NN GGCCGG^CC FseI CCGG GGCCNNNN^NGGCC SfiI NNN GGCGGA(11/9)^* EciI NN GGGCC^C ApaI GGCC GCSG^C TauI CSG GGTAC^C KpnI GTAC GGTGA(8/7)^* HphI N GKGCM^C BseSI KGCM GRGCY^C Eco24I RGCY GTATCC(6/5)^* BfuI N GTGCAG(16/14)^* BsgI NN GWGCW^C Alw21I WGCW RCATG^Y XceI CATG RGCGC^Y Bsp143II GCGC ^(7/8)TCACC* HphI N ^(9/11)TCCGCC* EciI NN ^(7/8)TCTTC* MboII N TTAAT^TAA PacI AT