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摘 要:摘要采用均匀设计安排实验,考察各影响因素与一组色谱分离评价函数间的综合关联程度。将关联度分析用于RP-HPLC同时测定多种头孢菌素的析因试验,从6个影响因素中确定主要影响因素。结果表明,乙腈配比、pH值、磷酸盐缓冲液浓度为主要影响因素。RP-HPLC同时测定头孢菌素时,关联度分析能更科学地确定色谱条件中的优化因子。 The Application of Correspondence Analysis toFactorial Experiment for Analysis of Cephalosporinwith Reversed Phase High Performance Liquid chromatography Zhu Shiqian(China
Pharmaceutical University, Nanjing 210009) Abstract:Correspondence
analysis was used for determining the important factors from the six
factors influencing analysis of cephalosporin in reversed phase-high
performance liquid chromatography (RP-HPLC). Experiments were planed by
U7(76),
then the synthetical correspondence between a series of optimizations
and the factors was calculated. Result indicated that that proportion of
organic solvent, pH and concentration of buffer were three most
important factors. It showed that the correspondence analysis was a
better method to determining the important factors in RP-HPLC |
作者单位:朱世谦(中国药科大学基础部分析化学教研室,南京
210009) 倪坤仪(中国药科大学基础部分析化学教研室,南京 210009) 吴振洁(厦门市药品检验所,厦门 561012) 李泽(中国药科大学基础部分析化学教研室,南京 210009) 张启国(厦门市药品检验所,厦门 561012) 参考文献: [1]Zhu Shiqian(朱世谦),Wu
Zhenjie(吴振洁),Ni Kunyi(倪坤仪). Chinese J. World Notes on
Antibiotics(国外医药抗生素分册),1998,19(6):428~432 收稿日期:2000年5月8日 修稿日期:2000年12月7日 出版日期:2001年4月1日 |
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