摘要： 用高效液相色谱法同时分析、示差折光检测器检测酿酒酵母发酵液中的葡萄糖、乙醇及甘 油。方法快速、灵敏、操作方便。同时利用高效液相色谱法对酿酒酵母细胞群体发酵初期的 代谢行为进行了初步研究。
关键词：高效液相色谱法；示差折光检测器；酿酒酵母；发酵
中图分类号：O658 文献标识码：A 文章编号：1000-8713(2000)02-0170-03
Simultaneous Analysis of Low Concentrations of Gluco se, Ethanol and Glycerol by High Performance Liquid Chromatographic Method
LI Wei-jun WEI Xin-gui CONG Wei OUYANG Fan
（State Key Laboratory of Biochemical Engineering,The Chinese Academy of Sciences, Beijing 100080, China）
Abstract: A method based on high performance liquid chromato graphy (HPLC) for the analysis of glucose, ethanol and glycerol simultaneously is presented. The baseline separation was achieved at room temperature(25-30 ℃) on a Beckman μ -Spherogel carbohydrate column (6.5 mm i.d.×30 cm) with H2SO4-H2O(0.5∶ 1 000 ,V/V) as mobile phase at a flow rate of 1.000 mL/min. All the substa nces were detected with the Beckman 186 Refractive Index Detector. Under these c onditions, distinct peaks of glucose, ethanol and glycerol were resolved within 15 min. The retention time in the particular condition was (6.04±0.04) min for glucose, (13.36±0.08) min for ethanol and(8.72±0.05) min for glycerol. The det ection limit for glucose, ethanol and glycerol were found to be 10－5g, 10 －4g and 10－5g respectively. A series of experiments have been perf ormed to investigate the glucose consumption and the ethanol, glycerol productio n in the random growth of Saccharomyces cerevisiae with the method above. Only 0 .5 mL sample was nee ded each time. In 30 hours after inoculation，the cell density increased from 1 06 mL－1 to 107 mL－1.It was found that the glucose concentra tion decreased approxima tely linearly after 8 hours, and ethanol and glycerol was detected after the del ay period as expected. The results show that the method proposed is rapid and co nvenient with enough accuracy. The method may be used to investigate the metabol ic behavior of yeast in the fermentation process at the early stage or detect th e slight changes of concentrations of some objective substances in particular cu lture experiments.
Key words: high performance liquid chromatography; refractive index detector; Saccharomyces cerevisiae; fermentation
来自：色谱 2000 Vol.18 No.2 P.170-172
作者：
2007-5-18