鱼肉中微囊藻毒素的高效液相色谱法分析

摘　要：应用反相高效液相色谱法分析了鱼肉中藻毒素的含量。用BDSC18色谱柱，以水(含1％三氟乙酸):甲醇=30:70(V/V)溶液为流动相，238nm紫外检测。鱼肉样品用甲醇-水和丙酮混合溶剂提取，经正已烷萃取后，将有机相弃去，水相用固相萃取柱净化后进行高效液相色谱分析。该法检测限为10ng/g，峰面积标准曲线在50～250ng范围内有良好线性关系，平均回收率为85.1％～88.2％。

关键词：反相高效液相色谱法；固相萃取；藻毒素；鱼肉

分类号：O657.7⁺2；O658.2　文献标识码：A
文章编号：1006-6144(2001)02-0117-03

Determination of Microcystins in Fish by High Performance Liquid Chromatography

ZHANG Wei-hao（Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 420073, China）　
JIN Li-na（Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 420073, China）　
XU Xiao-qin（Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 420073, China）　

Abstract：A reversed-phased high performance liquid chromatographic method for the determination of microcystins in fish muscle is described. A BDS C18 column was used with a mobile phase of water (containing 1％ trifluoroacetic acid):methanol=30:70(V/V). The wavelength 238 nm was selected. The silver carp muscle sample was extracted with a mixture of methanol-water and acetone. After extraction with hexane, the organic layer was discharged and the water phase was cleaned up using solid phased extraction columns before injection. The method is sensitive with detection limits of 10 ng/g, the linear range of the calibration curve of peak area was from 50 ng/g to 500 ng/g and the average recoveries of microcystins were 85.2％～88.10％.
Keywords：RP-HPLC; Solid extraction; Microcystin; Fish

参考文献：

［1］张维昊,徐小清,丘昌强.环境科学研究[J],2001,14(2)：61.
［2］SouriaS. Lancet[J], 1998, 352(4): 121.
［3］Water analyses. Anal. Chem. [J], 1999, 71(1): 181R.
［4］陈少涟,刘肖芳,胡传林等.水生生物学报[J],1990,14(1)：49.
［5］Lawton L A, Edwards C, Beattie K A et al. Nat Toxins[J], 1995, 3: 50.
［6］Campbell D L, Lawton L A, Beattie K A et al. Environ Toxicol Water Qual. [J], 1994, 9: 71.
［7］徐立红,陈加平,许建明等.环境科学学报[J],1999,19(5)：536.
［8］Metcalf J S, Bell S G,Codd G A. Wat. Res. [J], 2000, 34(10): 2761.
［9］Hormazabal V, Underdal B, Skulbeg O. J. Liq. Chrom. ＆Rel. Technol. [J] ,2000, 23(2): 185.

收稿日期：2000年10月23日

出版日期：2001年4月20日