B-受体阻剂及色谱法分析超临界甲醇聚聚对苯二甲酸乙二醇酯的产物

摘　要：在毛细管电泳仪上对12种β-受体阻滞剂和类似物的光学对映体进行了分离，研究了它们的拆分条件。使用0.1mol/L,pH3.0的磷酸-三乙醇胺缓冲液，20kV电压，12～36mmol/L羟丙基-β-环糊精作为手性识别试剂。其中11种β-受体阻滞剂及其类似物以及苯乙醇胺、胺酵素在50cm普通毛细管上获得了较好的手性分离效果，普罗勒沙的对映体在本条件下未获分离。同时发现，芳香环上取代基位置对迁移时间的影响有一定规律。

关键词：毛细管电泳，手性分离，β-受体阻滞剂与类似物

分类号：065　文献标识码：A

Chiral Separation of β-Blockers and Their Analogues by Capillary Electrophoresis

Wang Jianjun（State Kay Laboratory of Microbial Resources, Institute of Microbiology, Chine se Academy of Sciences, Beijing 100080）　
Zheng Guojun（State Kay Laboratory of Microbial Resources, Institute of Microbiology, Chine se Academy of Sciences, Beijing 100080）　
Wang Jialiang（State Kay Laboratory of Microbial Resources, Institute of Microbiology, Chine se Academy of Sciences, Beijing 100080）　
Yang Liu（State Kay Laboratory of Microbial Resources, Institute of Microbiology, Chine se Academy of Sciences, Beijing 100080）　
Wu Jin（State Kay Laboratory of Microbial Resources, Institute of Microbiology, Chine se Academy of Sciences, Beijing 100080）　
Sha Q ian（State Kay Laboratory of Microbial Resources, Institute of Microbiology, Chine se Academy of Sciences, Beijing 100080）　
Sun Wanru（State Kay Laboratory of Microbial Resources, Institute of Microbiology, Chine se Academy of Sciences, Beijing 100080）　

Abstract：Chiral separation conditions of 12 kinds of β-blockers and β-blocker analogues were investigated by capillary electrophoresis. 11 of them, phenylethnolamine and clenbuterol were separated successfully under optim um conditions: 0.1 mol/L, pH 3.0 phosphate-trihydroxyethylamine buffer and proper concentration of hydroxypropyl-β-cyclodextrin (12～36　mmol/L) at 20 　kV voltage in 50　cm unmodified capillaries, but enantiomers of pronethalol was not separated. It was also found that the position of substituting group took effect on migration time.
Keywords：Capillary electrophoresis, chiral separation, β-blocke rs , β-blocker analogues

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收稿日期：2000年7月15日

修稿日期：2000年9月18日

出版日期：2001年6月1日