关联度分析在反相高效液相色谱同时测定多种头孢菌素的析因试验中的应用

摘　要：摘要采用均匀设计安排实验，考察各影响因素与一组色谱分离评价函数间的综合关联程度。将关联度分析用于RP-HPLC同时测定多种头孢菌素的析因试验，从6个影响因素中确定主要影响因素。结果表明，乙腈配比、pH值、磷酸盐缓冲液浓度为主要影响因素。RP-HPLC同时测定头孢菌素时，关联度分析能更科学地确定色谱条件中的优化因子。
关键词：头孢菌素，反相高效液相色谱法，析因试验，关联度分析
分类号：O65　文献标识码：A

The Application of Correspondence Analysis toFactorial Experiment for Analysis of Cephalosporinwith Reversed Phase High Performance Liquid chromatography

Zhu Shiqian（China Pharmaceutical University, Nanjing 210009）　
Ni Kunyi（China Pharmaceutical University, Nanjing 210009）　
Wu Zhenjie（Xiamen Drug′s Control Institute）　
Li Ze（China Pharmaceutical University, Nanjing 210009）　
Zhang Qiguo（Xiamen Drug′s Control Institute）　

Abstract：Correspondence analysis was used for determining the important factors from the six factors influencing analysis of cephalosporin in reversed phase-high performance liquid chromatography (RP-HPLC). Experiments were planed by U7（76）, then the synthetical correspondence between a series of optimizations and the factors was calculated. Result indicated that that proportion of organic solvent, pH and concentration of buffer were three most important factors. It showed that the correspondence analysis was a better method to determining the important factors in RP-HPLC
Keywords：Cephalosporin, reversed phase-high performance liquid chromatography, factorial experiment, correspondence analysis

参考文献：

［1］Zhu Shiqian(朱世谦),Wu Zhenjie(吴振洁),Ni Kunyi(倪坤仪). Chinese J. World Notes on Antibiotics(国外医药抗生素分册),1998,19（6）：428～432
［2］Hu Yuzhu(胡育筑). Concise Tutorial of Chemometrics(化学计量学简明教程). Beijing(北京) ：Chinese Medicinal Technology Press(中国医药科技出版社),1997：66～69
［3］Mo Hua(莫华),Deng Bo(邓勃). Chinese J. Anal. Chem.(分析化学),1995,23（7）：779～782
［4］Wu Huifang(吴惠芳),Guan Fuyu(关福玉),Luo Yi(罗毅). Chinese J. Anal. Chem.(分析化学),1996,24（10）：1117～1122
［5］Hu Yuzhu(胡育筑). Concise Tutorial of Chemometrics(化学计量学简明教程). Beijing(北京): Chinese Medicinal Technology Press(中国医药科技出版社), 1997:1921 Zhu Shiqian(朱世谦),Wu Zhenjie(吴振洁),Ni Kunyi(倪坤仪). Chinese J. World Notes on Antibiotics(国外医药抗生素分册),1998,19（6）：428～432

收稿日期：2000年5月8日

修稿日期：2000年12月7日

出版日期：2001年4月1日